Overexpression of an AP2/ERF Type Transcription Factor OsEREBP1 Confers Biotic and Abiotic Stress Tolerance in Rice.
Overexpression of an AP2/ERF Type Transcription Factor OsEREBP1 Confers Biotic and Abiotic Stress Tolerance in Rice.
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DOI：
10.1371/journal.pone.0127831
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Ramanan R
中科院分区：
文献类型：
--
作者：
Jisha V;Dampanaboina L;Vadassery J;Mithöfer A;Kappara S;Ramanan R
AP 2/ERF类转录因子在植物生长发育和对环境刺激的反应中起着重要的调节作用。OsEREBP 1是水稻AP 2/ERF转录因子，是水稻与病原菌Xoo（Xanthomonasarumpv.）米）。OsEREBP 1在玉米泛素启动子驱动下在水稻中的组成型表达对植株的正常生长没有影响。基因芯片分析表明，OsEREBP 1的过表达导致脂酶、叶绿体脂氧合酶等脂质代谢相关基因以及茉莉酸和脱落酸生物合成相关基因的表达增加。PR基因、转录调节因子和Aldhs（酒精脱氢酶）在转基因植株中也被上调，这些基因与非生物胁迫和耐淹性有关。转基因植株表现出α-亚麻酸、几种茉莉酸衍生物和脱落酸的内源水平增加，但水杨酸没有增加。可溶性修饰的GFP（SmGFP）标记的OsEREBP 1定位于质体类核。对非转基因和OsEREBP 1过表达基因型的比较分析表明，OsEREBP 1减轻了Xoo引起的病害，并赋予转基因水稻耐旱和耐淹性。我们的研究结果表明，OsEREBP 1的组成型表达激活茉莉酸和脱落酸信号通路，从而引发水稻植株在非生物或生物胁迫条件下提高生存。OsEREBP 1是一个很好的候选基因，可用于植物抗逆工程。
AP2/ERF–type transcription factors regulate important functions of plant growth and development as well as responses to environmental stimuli. A rice AP2/ERF transcription factor, OsEREBP1 is a downstream component of a signal transduction pathway in a specific interaction between rice (Oryza sativa) and its bacterial pathogen, Xoo (Xanthomonas oryzae pv. oryzae). Constitutive expression of OsEREBP1 in rice driven by maize ubiquitin promoter did not affect normal plant growth. Microarray analysis revealed that over expression of OsEREBP1 caused increased expression of lipid metabolism related genes such as lipase and chloroplastic lipoxygenase as well as several genes related to jasmonate and abscisic acid biosynthesis. PR genes, transcription regulators and Aldhs (alcohol dehydrogenases) implicated in abiotic stress and submergence tolerance were also upregulated in transgenic plants. Transgenic plants showed increase in endogenous levels of α-linolenate, several jasmonate derivatives and abscisic acid but not salicylic acid. Soluble modified GFP (SmGFP)-tagged OsEREBP1 was localized to plastid nucleoids. Comparative analysis of non-transgenic and OsEREBP1 overexpressing genotypes revealed that OsEREBP1 attenuates disease caused by Xoo and confers drought and submergence tolerance in transgenic rice. Our results suggest that constitutive expression of OsEREBP1 activates the jasmonate and abscisic acid signalling pathways thereby priming the rice plants for enhanced survival under abiotic or biotic stress conditions. OsEREBP1 is thus, a good candidate gene for engineering plants for multiple stress tolerance.
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