Isolation from Proteus mirabilis of a novel class I 5-enopyruvylshikimate-3-phosphate synthase with glyphosate tolerance in transgenic Arabidopsis thaliana

Isolation from Proteus mirabilis of a novel class I 5-enopyruvylshikimate-3-phosphate synthase with glyphosate tolerance in transgenic Arabidopsis thaliana
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在转基因拟南芥中从奇异变形杆菌中分离出具有草甘膦耐受性的新型 I 类 5-烯丙酮莽草酸-3-磷酸合酶

DOI:
10.1007/s11738-013-1414-7
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发表时间:
2014-02
期刊:
Acta Physiol Plant
影响因子:
--
通讯作者:
姚泉洪
姚泉洪
中科院分区:
其他
文献类型:
--
作者:
姚泉洪

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应用基因组文库构建和菌落筛选的方法,从奇异变形杆菌中分离到一个编码5-烯丙酮基莽草酸-3-磷酸合成酶的新基因aroA。此外,还获得了新的aroA基因的转基因拟南芥,以证实aroA基因在培育抗草甘膦作物方面的潜力。
Applying the genomic library construction process and colony screening, a novel aroA gene encoding 5-enopyruvylshikimate-3-phosphate synthase from Proteus mirabilis was identified and isolated. Furthermore, the transgenic Arabidopsis with the novel aroA gene was obtained to confirm the potential of the novel aroA gene in developing glyphosate-resistant crops.
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