Dissecting the genetic control of natural variation in salt tolerance of Arabidopsis thaliana accessions.
Dissecting the genetic control of natural variation in salt tolerance of Arabidopsis thaliana accessions.
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DOI：
10.1093/jxb/erp376
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发表时间：
2010-02
影响因子：
6.9
通讯作者：
Taji T
中科院分区：
文献类型：
--
作者：
Katori T;Ikeda A;Iuchi S;Kobayashi M;Shinozaki K;Maehashi K;Sakata Y;Tanaka S;Taji T
已收集到许多拟南芥材料（生态型）。虽然它们之间的核苷酸序列差异很小，但这些细微的差异导致了表型性状（如抗逆性和开花时间）的巨大遗传变异。为了解拟南芥耐盐性的自然变异，对350份拟南芥材料进行了大规模盆栽试验。结果表明，不同品种的耐盐性差异较大。与盐敏感的试验品种Col-0相比，包括Bu-5、Bur-0、Ll-1、Wl-0和Zu-0在内的几个品种表现出明显的耐盐性。通过琼脂平板试验对耐盐品种进行了评价。通过大规模试验获得的数据与盐驯化（SA）试验的结果吻合良好，在盐驯化试验中，植物在中盐培养基上放置7 d后转移到高盐培养基。遗传分析表明，不含SA的耐盐性状是一种多基因控制的数量性状，而含SA的耐盐性状是由位于5号染色体上的单个基因调控的，这在显著耐盐品种中是常见的。这些结果为了解拟南芥耐盐性自然变异的机制提供了重要信息。
Many accessions (ecotypes) of Arabidopsis have been collected. Although few differences exist among their nucleotide sequences, these subtle differences induce large genetic variation in phenotypic traits such as stress tolerance and flowering time. To understand the natural variability in salt tolerance, large-scale soil pot experiments were performed to evaluate salt tolerance among 350 Arabidopsis thaliana accessions. The evaluation revealed a wide variation in the salt tolerance among accessions. Several accessions, including Bu-5, Bur-0, Ll-1, Wl-0, and Zu-0, exhibited marked stress tolerance compared with a salt-sensitive experimental accession, Col-0. The salt-tolerant accessions were also evaluated by agar plate assays. The data obtained by the large-scale assay correlated well with the results of a salt acclimation (SA) assay, in which plants were transferred to high-salinity medium following placement on moderate-salinity medium for 7 d. Genetic analyses indicated that the salt tolerance without SA is a quantitative trait under polygenic control, whereas salt tolerance with SA is regulated by a single gene located on chromosome 5 that is common among the markedly salt-tolerant accessions. These results provide important information for understanding the mechanisms underlying natural variation of salt tolerance in Arabidopsis.
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影响因子：
7.4
作者：
Kobayashi, Yuriko;Kuroda, Keishi;Koyama, Hiroyuki
通讯作者：
Koyama, Hiroyuki
DOI：
10.1007/bf00282217
发表时间：
1994-12-01
期刊：
MOLECULAR & GENERAL GENETICS
影响因子：
--
作者：
KOWALSKI, SP;LAN, TH;PATERSON, AH
通讯作者：
PATERSON, AH
影响因子：
11.6
作者：
Moller, Inge S.;Gilliham, Matthew;Tester, Mark
通讯作者：
Tester, Mark
影响因子：
7.4
作者：
MURPHY, A;TAIZ, L
通讯作者：
TAIZ, L
影响因子：
56.9
作者：
Apse, MP;Aharon, GS;Blumwald, E
通讯作者：
Blumwald, E