Molecular cloning and phylogenetic analysis of ORF7 region of chinese isolate TH-98 from transmissible gastroenteritis virus.
Molecular cloning and phylogenetic analysis of ORF7 region of chinese isolate TH-98 from transmissible gastroenteritis virus.
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来自可传染性胃炎病毒的中国分离物Th-98的ORF7区域的分子克隆和系统发育分析。
DOI：
10.1007/s11262-004-6783-y
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发表时间：
2005-05
期刊：
影响因子：
1.6
通讯作者：
Li YJ
中科院分区：
文献类型：
--
作者：
Yin JC;Ren XF;Li YJ
从猪传染性胃肠炎病毒（TGEV）中国分离株TH-98中提取基因组RNA。利用RT-PCR技术扩增TGEV的ORF 7序列，该序列位于TGEV基因组的3′端，目前对其功能尚不清楚。将ORF 7基因插入原核表达载体pPROEX HTc中，构建重组质粒pPROEX HTc-hp。对重组子进行测序，并与部分参考菌株进行比较。该基因全长237 bp，编码78个氨基酸，分子量为9.1 kDa，命名为hp基因（Genbank登录号：AY 337931）。与国外11株TGEV参考株比较，Hp基因和Hp蛋白的同源性分别为89%~ 97%和87%~ 96%，表明尽管ORF 7区相对保守，但株内变异较大。此外，基于这些ORF 7 DNA序列的系统发育树，树的拓扑结构表明，可能的重组事件发生在TGEV的进化历史。
Genomic RNA was extracted from a Chinese isolate of porcine transmissible gastroenteritis virus (TGEV) designated TH-98. Employing RT-PCR technique to amplify ORF7 sequence of TGEV, which located at the 3′ end of TGEV genome and is poorly understood functionally so far. A recombinant named pPROEX HTc-hp was constructed via inserting ORF7 gene into prokaryotic expression vector pPROEX HTc. The recombinant was sequenced and compared the DNA and its deduced amino acid (aa) sequences with that of some reference strains after restriction endonuclease and PCR analysis. The ORF7 gene named hp gene (Genbank accession number: AY337931) consists of 237 bp in length encoding a hydrophobic protein (HP) of 78 aa with a molecular weight of 9.1 kDa. The sequences of hp gene and Hp protein share 89%–97% and 87%–96% homologous identities compared with 11 TGEV reference strains derived from other regions or countries respectively, which revealed that there are significant variation within-strains, even though the ORF7 region is relatively conservative. In addition, a phylogenetic tree based on these ORF7 DNA sequences was generated, and the tree topology suggests that possible recombination events happened in the evolutionary history of TGEV.
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影响因子：
2.7
作者：
González JM;Gomez-Puertas P;Cavanagh D;Gorbalenya AE;Enjuanes L
通讯作者：
Enjuanes L
影响因子：
3.7
作者：
Ballesteros, ML;Sanchez, CM;Enjuanes, L
通讯作者：
Enjuanes, L
影响因子：
5.4
作者：
Schwegmann-Wessels, C;Zimmer, G;Herrler, G
通讯作者：
Herrler, G
影响因子：
3.7
作者：
Ortego J;Sola I;Almazán F;Ceriani JE;Riquelme C;Balasch M;Plana J;Enjuanes L
通讯作者：
Enjuanes L
影响因子：
1.6
作者：
Penzes Z;Gonzalez JM;Calvo E;Izeta A;Smerdou C;Méndez A;Sanchez CM;Sola I;Almazan F;Enjuanes L
通讯作者：
Enjuanes L