Expression profiling and comparative analyses of seven midgut serine proteases from the yellow fever mosquito, Aedes aegypti.
Expression profiling and comparative analyses of seven midgut serine proteases from the yellow fever mosquito, Aedes aegypti.
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DOI：
10.1016/j.jinsphys.2010.01.003
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发表时间：
2010-07
影响因子：
2.2
通讯作者：
Olson, Ken E.
中科院分区：
文献类型：
--
作者：
Brackney, Doug E.;Isoe, Jun;Black, W. C., IV;Zamora, Jorge;Foy, Brian D.;Miesfeld, Roger L.;Olson, Ken E.
埃及伊蚊利用血液来产生能量、卵成熟和补充母体储备。负责血粉消化的主要中肠酶是S1.A亚家族内的内切蛋白水解丝氨酸型蛋白酶。而A.在埃及伊蚊基因组中，已知只有五种在中肠中表达。我们描述了7个额外的丝氨酸蛋白酶的克隆，测序和表达谱，并提供了这些发现的基因组和系统发育评估。在这七个基因中，四个是组成型表达的，三个是在吸血后转录诱导的。转录诱导的量在这些基因之间强烈相关。比对显示，在一般情况下，保守的催化三联体，活性位点和辅助催化残基保持在这些基因和系统发育分析表明，这些基因属于三个不同的分支，胰蛋白酶，胰凝乳蛋白酶和丝氨酸胶原酶。有趣的是，以前描述的胰蛋白酶一致出现与其他丝氨酸胶原酶的系统发育分析。这些结果表明，在中肠丝氨酸蛋白酶的S1.A亚家族中已经出现了多个基因重复，并且/或者A.埃及人已经进化出一系列具有广泛底物特异性的蛋白酶，用于快速、有效地消化血粉。
Aedes aegypti utilizes blood for energy production, egg maturation and replenishment of maternal reserves. The principle midgut enzymes responsible for bloodmeal digestion are endoproteolytic serine-type proteases within the S1.A subfamily. While there are hundreds of serine protease-like genes in the A. aegypti genome, only five are known to be expressed in the midgut. We describe the cloning, sequencing and expression profiling of seven additional serine proteases and provide a genomic and phylogenetic assessment of these findings. Of the seven genes, four are constitutively expressed and three are transcriptionally induced upon blood feeding. The amount of transcriptional induction is strongly correlated among these genes. Alignments reveal that, in general, the conserved catalytic triad, active site and accessory catalytic residues are maintained in these genes and phylogenetic analysis shows that these genes fall within three distinct clades; trypsins, chymotrypsins and serine collagenases. Interestingly, a previously described trypsin consistently arose with other serine collagenases in phylogenetic analyses. These results suggest that multiple gene duplications have arisen within the S1.A subfamily of midgut serine proteases and/or that A. aegypti has evolved an array of proteases with a broad range of substrate specificities for rapid, efficient digestion of bloodmeals.
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影响因子：
56.9
作者：
NEURATH, H
通讯作者：
NEURATH, H
影响因子：
4.4
作者：
Dana AN;Hong YS;Kern MK;Hillenmeyer ME;Harker BW;Lobo NF;Hogan JR;Romans P;Collins FH
通讯作者：
Collins FH
DOI：
10.1111/j.1432-1033.1985.tb09171.x
发表时间：
1985-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
LECROISEY, A;KEIL, B
通讯作者：
KEIL, B
影响因子：
3.8
作者：
Bian, Guowu;Ralkhel, Alexander S.;Zhu, Jinsong
通讯作者：
Zhu, Jinsong
影响因子：
10.7
作者：
SAITOU, N;NEI, M
通讯作者：
NEI, M