Gene expression patterns associated with blood-feeding in the malaria mosquito Anopheles gambiae.
Gene expression patterns associated with blood-feeding in the malaria mosquito Anopheles gambiae.
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DOI：
10.1186/1471-2164-6-5
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发表时间：
2005-01-14
期刊：
影响因子：
4.4
通讯作者：
Collins FH
中科院分区：
文献类型：
--
作者：
Dana AN;Hong YS;Kern MK;Hillenmeyer ME;Harker BW;Lobo NF;Hogan JR;Romans P;Collins FH
吸血或噬血是雌蚊繁殖和传播病原体所必需的一种行为。我们利用cDNA微阵列测定了3068条ESTs的表达模式，这些ESTs代表了大约2000个独特的基因转录本，分别在摄入血液后的头两天、40分钟血餐期间的5分钟和30分钟以及完成血餐后的0、1、3、5、12、16、24和48小时在成年雌性冈比亚按蚊中的表达模式，并将它们的表达水平与仅使用糖溶液的蚊子的表达水平进行了比较。在血液喂养的蚊子中，413个独特转录本（约占总数的25%）在一个或多个时间点上的表达量比糖喂养的蚊子高出或低于其水平至少两倍。使用k-means聚类将这些差异表达的基因产物聚类为Early Genes、Middle Genes和Late Genes，分别包含144、130和139个独特转录本。通过实时荧光定量PCR对每组的几个基因进行分析，以验证微阵列结果。本文结合雌蚊对血液摄食的生理反应，包括血液消化、营养基质形成、卵发育和免疫等，讨论了这三组基因（早、中、晚）的表达模式和注释。
Blood feeding, or hematophagy, is a behavior exhibited by female mosquitoes required both for reproduction and for transmission of pathogens. We determined the expression patterns of 3,068 ESTs, representing ~2,000 unique gene transcripts using cDNA microarrays in adult female Anopheles gambiae at selected times during the first two days following blood ingestion, at 5 and 30 min during a 40 minute blood meal and at 0, 1, 3, 5, 12, 16, 24 and 48 hours after completion of the blood meal and compared their expression to transcript levels in mosquitoes with access only to a sugar solution. In blood-fed mosquitoes, 413 unique transcripts, approximately 25% of the total, were expressed at least two-fold above or below their levels in the sugar-fed mosquitoes, at one or more time points. These differentially expressed gene products were clustered using k-means clustering into Early Genes, Middle Genes, and Late Genes, containing 144, 130, and 139 unique transcripts, respectively. Several genes from each group were analyzed by quantitative real-time PCR in order to validate the microarray results. The expression patterns and annotation of the genes in these three groups (Early, Middle, and Late genes) are discussed in the context of female mosquitoes' physiological responses to blood feeding, including blood digestion, peritrophic matrix formation, egg development, and immunity.
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影响因子：
64.5
作者：
Cha, BJ;Koppetsch, BS;Theurkauf, WE
通讯作者：
Theurkauf, WE
影响因子：
4.8
作者：
Brown, MR;Graf, R;Lea, AO
通讯作者：
Lea, AO
DOI：
10.1016/0020-1790(85)90094-0
发表时间：
1985-01-01
期刊：
INSECT BIOCHEMISTRY
影响因子：
--
作者：
CHEESEMAN, MT;GOODING, RH
通讯作者：
GOODING, RH
影响因子：
3.8
作者：
BARILLASMURY, CV;NORIEGA, FG;WELLS, MA
通讯作者：
WELLS, MA
影响因子：
2.2
作者：
BILLINGSLEY, PF
通讯作者：
BILLINGSLEY, PF