Ammonia-Oxidizing Archaea Are More Resistant Than Denitrifiers to Seasonal Precipitation Changes in an Acidic Subtropical Forest Soil.
Ammonia-Oxidizing Archaea Are More Resistant Than Denitrifiers to Seasonal Precipitation Changes in an Acidic Subtropical Forest Soil.
复制标题
氨氧化古菌比反硝化菌更能抵抗酸性亚热带森林土壤中的季节性降水变化
DOI：
10.3389/fmicb.2017.01384
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发表时间：
2017
影响因子：
5.2
通讯作者：
Shen W
中科院分区：
文献类型：
--
作者：
Chen J;Nie Y;Liu W;Wang Z;Shen W
亚热带地区降水季节变化越来越剧烈。然而，土壤氮循环及其相关功能微生物对降水变化的响应尚不清楚。在这项研究中，两个预测的降水模式进行了操纵：加强旱季干旱（DD）和延长旱季持续时间（艾德），但增加雨季风暴后DD和艾德治疗期间。通过抗性指数定量评价了这两种降水模式对土壤净氮转化率和功能基因丰度的影响。结果表明，在整个试验过程中，功能微生物丰度的阻力指数（-0.03 ± 0.08）远低于净氮转化率的阻力指数（0.55 ± 0.02），表明微生物丰度比净氮转化率对降水变化的响应更敏感。春季干旱下的艾德治疗显着增加了硝化（amoA）和硝化基因（nirK，nirS，和nosZ）的丰度，而这些基因丰度的变化在很大程度上重叠在旱季干旱期间与对照处理。有趣的是，氨氧化古菌amoA丰度的抗性指数显著高于反硝化基因丰度的抗性指数，表明氨氧化古菌对降水变化的抗性更强。这是由于AOA群落具有较强的环境适应性和较高的资源利用效率，如AOA基因丰度与环境因子之间缺乏相关性[即，土壤含水量、铵态氮（NH 4+）和溶解性有机碳浓度]。
Seasonal precipitation changes are increasingly severe in subtropical areas. However, the responses of soil nitrogen (N) cycle and its associated functional microorganisms to such precipitation changes remain unclear. In this study, two projected precipitation patterns were manipulated: intensifying the dry-season drought (DD) and extending the dry-season duration (ED) but increasing the wet-season storms following the DD and ED treatment period. The effects of these two contrasting precipitation patterns on soil net N transformation rates and functional gene abundances were quantitatively assessed through a resistance index. Results showed that the resistance index of functional microbial abundance (-0.03 ± 0.08) was much lower than that of the net N transformation rate (0.55 ± 0.02) throughout the experiment, indicating that microbial abundance was more responsive to precipitation changes compared with the N transformation rate. Spring drought under the ED treatment significantly increased the abundances of both nitrifying (amoA) and denitrifying genes (nirK, nirS, and nosZ), while changes in these gene abundances overlapped largely with control treatment during droughts in the dry season. Interestingly, the resistance index of the ammonia-oxidizing archaea (AOA) amoA abundance was significantly higher than that of the denitrifying gene abundances, suggesting that AOA were more resistant to the precipitation changes. This was attributed to the stronger environmental adaptability and higher resource utilization efficiency of the AOA community, as indicated by the lack of correlations between AOA gene abundance and environmental factors [i.e., soil water content, ammonium (NH4+) and dissolved organic carbon concentrations] during the experiment.
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DOI：
10.1073/pnas.1109000108
发表时间：
2011-12-27
影响因子：
11.1
作者：
Gubry-Rangin, Cecile;Hai, Brigitte;Nicol, Graeme W.
通讯作者：
Nicol, Graeme W.
影响因子：
11.6
作者：
Bell, Colin W.;Tissue, David T.;Zak, John C.
通讯作者：
Zak, John C.
影响因子：
11
作者：
Bouskill, Nicholas J.;Lim, Hsiao Chien;Brodie, Eoin L.
通讯作者：
Brodie, Eoin L.
影响因子：
11.6
作者：
Allison, Steven D.;Treseder, Kathleen K.
通讯作者：
Treseder, Kathleen K.
影响因子：
3.6
作者：
Griffiths, BS;Kuan, HL;Fenwick, C
通讯作者：
Fenwick, C