Human mesenchymal stem cells express neuronal markers after osteogenic and adipogenic differentiation.
Human mesenchymal stem cells express neuronal markers after osteogenic and adipogenic differentiation.
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DOI：
10.2478/s11658-013-0083-2
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发表时间：
2013-06
影响因子：
8.3
通讯作者：
Miloso M
中科院分区：
文献类型：
--
作者：
Foudah D;Redondo J;Caldara C;Carini F;Tredici G;Miloso M
间充质干细胞（MSC）是多能细胞，其能够分化成中胚层谱系（成骨、成脂、成软骨），但也朝向非中胚层衍生物（例如神经细胞）分化。最近的体外研究表明，在没有任何种类的分化刺激的情况下，未分化的MSC表达神经分化标志物，但文献数据并不完全一致。考虑到它们对神经退行性疾病的有希望的治疗潜力，扩大我们对MSC这种特殊生物学特性的了解是非常重要的。在这项研究中，我们证实了未分化的人MSC（hMSC）自发表达神经标志物（神经元，神经胶质和祖细胞标志物），特别是，我们证明了神经元标志物β III-微管蛋白和NeuN由非常高百分比的hMSC表达，无论培养传代次数和培养条件如何。此外，神经元标记物β III-微管蛋白和NeuN在体外成骨和成脂分化后仍由hMSCs表达。另一方面，软骨分化的hMSCs对这些标记物呈阴性。我们的研究结果表明，神经元标志物的表达可能是常见的广泛的细胞类型，而不是排他性的神经元谱系。因此，单独的神经元标志物的表达不足以证明MSC向神经元表型的分化。还需要进行功能特性分析。
Mesenchymal stem cells (MSCs) are multipotent cells that are able to differentiate into mesodermal lineages (osteogenic, adipogenic, chondrogenic), but also towards non-mesodermal derivatives (e.g. neural cells). Recent in vitro studies revealed that, in the absence of any kind of differentiation stimuli, undifferentiated MSCs express neural differentiation markers, but the literature data do not all concur. Considering their promising therapeutic potential for neurodegenerative diseases, it is very important to expand our knowledge about this particular biological property of MSCs. In this study, we confirmed the spontaneous expression of neural markers (neuronal, glial and progenitor markers) by undifferentiated human MSCs (hMSCs) and in particular, we demonstrated that the neuronal markers βIII-tubulin and NeuN are expressed by a very high percentage of hMSCs, regardless of the number of culture passages and the culture conditions. Moreover, the neuronal markers βIII-tubulin and NeuN are still expressed by hMSCs after in vitro osteogenic and adipogenic differentiation. On the other hand, chondrogenically differentiated hMSCs are negative for these markers. Our findings suggest that the expression of neuronal markers could be common to a wide range of cellular types and not exclusive for neuronal lineages. Therefore, the expression of neuronal markers alone is not sufficient to demonstrate the differentiation of MSCs towards the neuronal phenotype. Functional properties analysis is also required.
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影响因子：
3.7
作者：
Dredge BK;Jensen KB
通讯作者：
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影响因子：
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作者：
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作者：
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通讯作者：
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影响因子：
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作者：
Gilyarov, A V
通讯作者：
Gilyarov, A V