LPS nephropathy in mice is ameliorated by IL-2 independently of regulatory T cells activity.
LPS nephropathy in mice is ameliorated by IL-2 independently of regulatory T cells activity.
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DOI：
10.1371/journal.pone.0111285
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Ghiggeri GM
中科院分区：
文献类型：
--
作者：
Bertelli R;Di Donato A;Cioni M;Grassi F;Ikehata M;Bonanni A;Rastaldi MP;Ghiggeri GM
Immunosuppressive regulatory T cells (Tregs) have been hypothesized to exert a protective role in animal models of spontaneous (Buffalo/Mna) and/or drug induced (Adriamycin) nephrotic syndrome. In this study, we thought to define whether Tregs can modify the outcome of LPS nephropathy utilizing IL-2 as inducer of tissue and circulating Tregs. LPS (12 mg/Kg) was given as single shot in C57BL/6, p2rx7−/− and Foxp3EGFP; free IL-2 (18.000 U) or, in alternative, IL-2 coupled with JES6-1 mAb (IL-2/anti-IL-2) were injected before LPS. Peripheral and tissue Tregs/total CD4+ cell ratio, urinary parameters and renal histology were evaluated for 15 days. IL-2 administration to wild type mice had no effect on peripheral Tregs number, whereas a significant increase was induced by the IL-2/anti-IL-2 immunocomplex after 5 days. Spleen and lymph nodes Tregs were comparably increased. In p2rx7−/− mice, IL-2/anti-IL-2 treatment resulted in increase of peripheral Tregs but did not modify the spleen and lymph nodes quota. LPS induced comparable and transient proteinuria in both wild type and p2rx7−/− mice. Proteinuria was inhibited by co-infusion of human IL-2, with reduction at each phase of the disease (24 −48 and 72 hours) whereas IL-2/anti-IL-2 produced weaker effects. In all mice (wild type and p2rx7−/−) and irrespective of treatment (IL-2, IL-2/anti-IL-2), LPS was associated with progressive signs of renal pathologic involvement resulting in glomerulosclerosis. In conclusion, IL-2 plays a transient protective effect on proteinuria induced by LPS independent of circulating or tissue Tregs but does not modify the outcome of renal degenerative renal lesions.
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影响因子：
6.1
作者：
Caridi, Gianluca;Lugani, Francesca;Ghiggeri, Gian Marco
通讯作者：
Ghiggeri, Gian Marco
影响因子：
13.6
作者：
Musante, Luca;Candiano, Giovanni;Ghiggeri, Gian Marco
通讯作者：
Ghiggeri, Gian Marco
影响因子：
13.2
作者：
Ghiggeri, GM;Caridi, G;Balduini, CL
通讯作者：
Balduini, CL
DOI：
10.2741/3657
发表时间：
2010-06-01
期刊：
Frontiers in bioscience (Landmark edition)
影响因子：
--
作者：
Mai J;Wang H;Yang XF
通讯作者：
Yang XF
影响因子：
6
作者：
Geertsen, PF;von der Maase, H;Leyssac, PP
通讯作者：
Leyssac, PP