Comparative analysis of the composition of intestinal bacterial communities in Dastarcus helophoroides fed different diets
Comparative analysis of the composition of intestinal bacterial communities in Dastarcus helophoroides fed different diets
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不同日粮饲喂的大蠹肠道细菌群落组成比较分析
DOI：
10.1093/jis/14.1.111
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发表时间：
2014-08
影响因子：
2.2
通讯作者：
Meng-Lou Li
中科院分区：
文献类型：
--
作者：
Cai He;Jun Cui;Hai-Dong Wang;Meng-Lou Li
摘要采用PCR-DGGE技术研究花绒寄甲幼虫和成虫肠道细菌群落的多样性，以探讨不同人工饲料对花绒寄甲肠道细菌群落的影响。两种饲料用于喂养幼虫和四个成人。从成虫肠道中分离到埃希氏菌属、葡萄球菌属、葡萄球菌属、囊茎星状菌属、纤维弧菌属、金黄色单胞菌属和浮游微菌属，其中埃希氏菌属和葡萄球菌属是主要的细菌群落，且不同饵料的成虫肠道细菌数量不同。具体而言，饲喂不同饲料的成虫肠道细菌数量按饲料的主要成分排序为：蚂蚁粉>黑翅虫蛹粉>蟋蟀粉>蚕蛹粉。从幼虫的肠道中分离出埃希氏菌、芽孢杆菌、葡萄球菌、克氏菌、Planococcaceae、罗尔斯通氏菌、纤发菌、不动杆菌和假单胞菌。饲喂拟步甲蛹的幼虫肠道细菌数量高于饲喂其他人工饲料的幼虫。本研究首次研究了人工饲料对罗非鱼肠道细菌群落和微生物多样性的影响。花绒草属
Abstract The diversity of the intestinal bacterial communities in Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae) larvae and adults was assayed by PCR-DGGE to determine whether different artificial diets could influence these bacterial communities. Two diets were used for feeding the larvae and four for the adults. Escherichia, Desemzia, Staphylococcus, Asticcacaulis, Cellvibrio, Aurantimonas, and Planomicrobium were isolated from the gut of the adults, with Escherichia and Staphylococcus being the main bacterial communities, and the quantities of intestinal bacterial were different in the adults fed different diets. Specifically, the amount of intestinal bacteria from the adults fed different diets had the following ranking according to the major component of the diet: ant powder > darkling beetle pupa powder > cricket powder > silkworm pupa powder. Escherichia, Bacillus, Staphylococcus, Kurthia, Planococcaceae, Ralstonia, Leptothrix, Acinetobacter, and Pseudomonas were isolated from the gut of the larvae. The quantity of intestinal bacteria from the larvae fed the darkling beetle pupae was greater than that from the larvae fed other artificial diets. This study, for the first time, investigated the effect of artificial diets on the bacterial community and the intestinal microbial diversity of D. helophoroides.
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影响因子：
3.4
作者：
Dillon, R. J.;Webster, G.;Charnley, A. K.
通讯作者：
Charnley, A. K.
影响因子：
3.7
作者：
HUGHES, DS;POSSEE, RD;KING, LA
通讯作者：
KING, LA
DOI：
--
发表时间：
2007
期刊：
--
影响因子：
--
作者：
X. Hui
通讯作者：
X. Hui
DOI：
--
发表时间：
2001-12
期刊：
Wei sheng wu xue bao = Acta microbiologica Sinica
影响因子：
--
作者：
Z. He;Y. Yin;Y. Cao;Y. Dong;W. Zhang
通讯作者：
Z. He;Y. Yin;Y. Cao;Y. Dong;W. Zhang
影响因子：
--
作者：
M. Kaufman;E. Walker;D. Odelson;M. Klug
通讯作者：
M. Kaufman;E. Walker;D. Odelson;M. Klug