Probing the growth and mechanical properties of Bacillus subtilis biofilms through genetic mutation strategies.
Probing the growth and mechanical properties of Bacillus subtilis biofilms through genetic mutation strategies.
复制标题
通过基因突变策略探讨枯草芽孢杆菌生物膜的生长和机械特性
DOI：
10.1016/j.synbio.2022.05.005
复制
发表时间：
2022-09
影响因子：
4.8
通讯作者：
Zhong, Chao
中科院分区：
文献类型：
--
作者：
Liu, Suying;Huang, Jiaofang;Zhang, Chen;Wang, Lihua;Fan, Chunhai;Zhong, Chao
细菌群落通过合成具有凝聚力和保护性的细胞外基质，在各种表面形成生物膜，这些生物膜保护微生物免受恶劣环境条件的侵害。枯草芽孢杆菌是一种应用广泛的实验物种，其生物膜被作为有益生物膜的代表性模型。具体来说，已知枯草芽孢杆菌生物膜富含细胞外聚合物质（EPS）和其他生物聚合物，如DNA和淀粉样蛋白TasA和疏水蛋白BslA等蛋白质。这些材料形成了一个相互连接的、有凝聚力的三维聚合物网络，提供了生物膜的机械稳定性，并在其他功能贡献中调节了它们对表面的粘附。在这里，我们探索了基因编码成分如何特异性地参与调节枯草芽孢杆菌生物膜的生长状态、机械特性和抗生素耐药性，从而为了解各种基因工程如何影响生物膜的结构和功能奠定了坚实的经验基础。我们注意到主要生物膜成分（如EPS、TasA和BslA）对生物膜形态、机械性能和存活的不同贡献。例如，EPS在维持生物膜的机械性能稳定性和抗生素耐药性方面起着重要作用，而BslA对打印应用中可以获得的分辨率有重要影响。这项工作通过系统的遗传操作，对生物膜组分的内部相互作用提供了更深入的了解。这不仅拓宽了有益生物膜的应用前景，也为未来靶向和有效去除有害生物膜的策略奠定了基础。
Bacterial communities form biofilms on various surfaces by synthesizing a cohesive and protective extracellular matrix, and these biofilms protect microorganisms against harsh environmental conditions. Bacillus subtilis is a widely used experimental species, and its biofilms are used as representative models of beneficial biofilms. Specifically, B. subtilis biofilms are known to be rich in extracellular polymeric substances (EPS) and other biopolymers such as DNA and proteins like the amyloid protein TasA and the hydrophobic protein BslA. These materials, which form an interconnected, cohesive, three-dimensional polymer network, provide the mechanical stability of biofilms and mediate their adherence to surfaces among other functional contributions. Here, we explored how genetically-encoded components specifically contribute to regulate the growth status, mechanical properties, and antibiotic resistance of B. subtilis biofilms, thereby establishing a solid empirical basis for understanding how various genetic engineering efforts are likely to affect the structure and function of biofilms. We noted discrete contributions to biofilm morphology, mechanical properties, and survival from major biofilm components such as EPS, TasA and BslA. For example, EPS plays an important role in maintaining the stability of the mechanical properties and the antibiotic resistance of biofilms, whereas BslA has a significant impact on the resolution that can be obtained for printing applications. This work provides a deeper understanding of the internal interactions of biofilm components through systematic genetic manipulations. It thus not only broadens the application prospects of beneficial biofilms, but also serves as the basis of future strategies for targeting and effectively removing harmful biofilms.
登录
查看更多内容
DOI：
10.1073/pnas.1718102115
发表时间：
2018-03-27
影响因子：
11.1
作者：
Diehl A;Roske Y;Ball L;Chowdhury A;Hiller M;Molière N;Kramer R;Stöppler D;Worth CL;Schlegel B;Leidert M;Cremer N;Erdmann N;Lopez D;Stephanowitz H;Krause E;van Rossum BJ;Schmieder P;Heinemann U;Turgay K;Akbey Ü;Oschkinat H
通讯作者：
Oschkinat H
影响因子：
4.7
作者：
Balasubramanian, Srikkanth;Aubin-Tam, Marie-Eve;Meyer, Anne S.
通讯作者：
Meyer, Anne S.
影响因子：
3.6
作者：
Cairns LS;Hobley L;Stanley-Wall NR
通讯作者：
Stanley-Wall NR
影响因子：
3.6
作者：
Chu, Frances;Kearns, Daniel B.;Losick, Richard
通讯作者：
Losick, Richard
影响因子：
3.4
作者：
Douarche, Carine;Allain, Jean-Marc;Raspaud, Eric
通讯作者：
Raspaud, Eric