ZmCTLP1 is required for the maintenance of lipid homeostasis and the basal endosperm transfer layer in maize kernels.
ZmCTLP1 is required for the maintenance of lipid homeostasis and the basal endosperm transfer layer in maize kernels.
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ZmCTLP1 是维持玉米籽粒中脂质稳态和基底胚乳转移层所必需的。
DOI：
10.1111/nph.17754
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发表时间：
2021-12
期刊：
影响因子：
9.4
通讯作者：
Song, Weibin
中科院分区：
文献类型：
--
作者：
Hu, Mingjian;Zhao, Haiming;Yang, Bo;Yang, Shuang;Liu, Haihong;Tian, He;Shui, Guanghou;Chen, Zongliang;Lizhu, E.;Lai, Jinsheng;Song, Weibin
玉米籽粒重量受母体胎座营养物质卸载及其通过胚乳基部转移层（BETL）和基部中间区（BIZ）的转移组织到达胚乳上部的影响。在这里，我们发现小内核10（Smk10）编码胆碱转运蛋白样蛋白1（ZmCTLP 1），促进胆碱摄取，位于反式高尔基体网络（TGN）中。其功能的丧失导致胆碱含量降低，导致淀粉含量较低的较小籽粒。ZmCTLP 1突变破坏膜脂质稳态和壁内生长的正常发育。两个籽粒灌浆相关基因Mn1和ZmSWEET4c在smk10中表达水平下调，这可能是转移细胞分化不完全的主要原因之一。ZmCTLP 1的突变也减少了转移细胞中的胞间连丝（PD）的数量，表明smk10突变体在PD形成中受损。有趣的是，我们还观察到smk10突变体的BETL和BIZ中的过早细胞死亡。总之，我们的结果表明，ZmCTLP 1介导的胆碱转运影响籽粒发育，突出了其在转移细胞中的脂质稳态，壁内生长形成和PD发育中的重要作用。
Maize kernel weight is influenced by the unloading of nutrients from the maternal placenta and their passage through the transfer tissue of the basal endosperm transfer layer (BETL) and the basal intermediate zone (BIZ) to the upper part of the endosperm. Here, we show that Small kernel 10 (Smk10) encodes a choline transporter‐like protein 1 (ZmCTLP1) that facilitates choline uptake and is located in the trans‐Golgi network (TGN). Its loss of function results in reduced choline content, leading to smaller kernels with a lower starch content. Mutation of ZmCTLP1 disrupts membrane lipid homeostasis and the normal development of wall in‐growths. Expression levels of Mn1 and ZmSWEET4c, two kernel filling‐related genes, are downregulated in the smk10, which is likely to be one of the major causes of incompletely differentiated transfer cells. Mutation of ZmCTLP1 also reduces the number of plasmodesmata (PD) in transfer cells, indicating that the smk10 mutant is impaired in PD formation. Intriguingly, we also observed premature cell death in the BETL and BIZ of the smk10 mutant. Together, our results suggest that ZmCTLP1‐mediated choline transport affects kernel development, highlighting its important role in lipid homeostasis, wall in‐growth formation and PD development in transfer cells.
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影响因子：
5.6
作者：
Bihmidine S;Hunter CT 3rd;Johns CE;Koch KE;Braun DM
通讯作者：
Braun DM
影响因子：
11.6
作者：
Doll, Nicolas M.;Just, Jeremy;Widiez, Thomas
通讯作者：
Widiez, Thomas
影响因子：
4.1
作者：
Clegg, K. M.
通讯作者：
Clegg, K. M.
影响因子：
1.5
作者：
Gao, R;Dong, S;Hu, C
通讯作者：
Hu, C
DOI：
10.1016/b978-0-12-811971-6.00012-7
发表时间：
2019-01-01
期刊：
CORN: CHEMISTRY AND TECHNOLOGY, 3RD EDITION
影响因子：
--
作者：
Larkins, Brian A.
通讯作者：
Larkins, Brian A.