Systematic identification and expression profiles of the BAHD superfamily acyltransferases in barley (Hordeum vulgare).
Systematic identification and expression profiles of the BAHD superfamily acyltransferases in barley (Hordeum vulgare).
复制标题
大麦 (Hordeum vulgare) 中 BAHD 超家族酰基转移酶的系统鉴定和表达谱
DOI：
10.1038/s41598-022-08983-7
复制
发表时间：
2022-03-24
影响因子：
4.6
通讯作者：
Xu D
中科院分区：
文献类型：
--
作者：
Yuan Z;Yang H;Pan L;Zhao W;Liang L;Gatera A;Tucker MR;Xu D
BAHD超家族酰基转移酶在催化和调节植物次生代谢中起重要作用。尽管如此，关于大麦中BAHD超家族的信息相对较少。在这项研究中，我们从大麦基因组中鉴定出116个HvBAHD酰基转移酶。通过对单子叶和双子叶模式植物的系统发育分析和分类，将这些基因分为I-a、I-b、II、III-a、III-b、IV、V-a和V-b 8个类群。在拟南芥中不存在与植物对赤霉素抗性相关的Agmatine Coumarol Transferase （ACT）等进化枝IV基因。HvBAHDs的顺式调控元件分析显示，这些基因对GA3处理反应积极。in -silico表达和qPCR分析显示，HvBAHD基因在多个组织和发育阶段均有表达，且在苗期高度富集，具有多种作用。单核苷酸多态性（SNP）扫描分析表明，HvBAHDs编码区的自然变异较低，且在大麦驯化过程中具有保守性。我们的研究结果揭示了HvBAHDs的复杂性，并将有助于在进一步的研究中对其进行分析。
BAHD superfamily acyltransferases play an important role in catalyzing and regulating secondary metabolism in plants. Despite this, there is relatively little information regarding the BAHD superfamily in barley. In this study, we identified 116 HvBAHD acyltransferases from the barley genome. Based on phylogenetic analysis and classification in model monocotyledonous and dicotyledonous plants, we divided the genes into eight groups, I-a, I-b, II, III-a, III-b, IV, V-a and V-b. The Clade IV genes, including Agmatine Coumarol Transferase (ACT) that is associated with resistance of plants to Gibberella fungi, were absent in Arabidopsis. Cis-regulatory element analysis of the HvBAHDs showed that the genes respond positively to GA3 treatment. In-silico expression and qPCR analysis showed the HvBAHD genes are expressed in a range of tissues and developmental stages, and highly enriched in the seedling stage, consistent with diverse roles. Single nucleotide polymorphism (SNP) scanning analysis revealed that the natural variation in the coding regions of the HvBAHDs is low and the sequences have been conserved during barley domestication. Our results reveal the complexity of the HvBAHDs and will help facilitate their analysis in further studies.
登录
查看更多内容
大麦 (Hordeum vulgare L.) 中 APETALA2/乙烯反应因子 (AP2/ERF) 基因家族的全基因组分析
影响因子：
3.7
作者：
Guo B;Wei Y;Xu R;Lin S;Luan H;Lv C;Zhang X;Song X;Xu R
通讯作者：
Xu R
影响因子：
7.2
作者：
Grienenberger, Etienne;Besseau, Sebastien;Legrand, Michel
通讯作者：
Legrand, Michel
影响因子：
7.4
作者：
Kosma, Dylan K.;Molina, Isabel;Pollard, Mike
通讯作者：
Pollard, Mike
影响因子：
7.4
作者：
Akhunov, Eduard D.;Sehgal, Sunish;Gill, Bikram S.
通讯作者：
Gill, Bikram S.
影响因子：
11.6
作者：
Hoffmann, L;Besseau, S;Legrand, M
通讯作者：
Legrand, M