Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation.
Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation.
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DOI：
10.1038/s41522-022-00272-5
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发表时间：
2022-02-25
影响因子：
9.2
通讯作者：
Garnett JA
中科院分区：
文献类型：
--
作者：
Corsini PM;Wang S;Rehman S;Fenn K;Sagar A;Sirovica S;Cleaver L;Edwards-Gayle CJC;Mastroianni G;Dorgan B;Sewell LM;Lynham S;Iuga D;Franks WT;Jarvis J;Carpenter GH;Curtis MA;Bernadó P;Darbari VC;Garnett JA
大肠杆菌是一种革兰氏阴性细菌，定植于人体肠道，毒力菌株可引起严重的肠道和肠外疾病。SslE蛋白是由一系列致病性大肠杆菌和大肠杆菌分泌的。大肠杆菌菌株。它可以降解肠道中的粘蛋白，促进生物膜成熟，并且它是强毒株感染的主要决定因素，尽管它如何执行这些功能尚不清楚。在这里，我们检查了来自Escherosal E的SslE。大肠杆菌Waksman和BL 21（DE 3）菌株以及肠致病性H10407和肠致病性E2348/69菌株。我们揭示了SslE在溶液中具有独特的动态结构，并且响应于成熟生物膜内的酸化，它可以形成具有淀粉样性质的独特聚集体。此外，我们表明，SslE单体和聚集体结合DNA在体外和共定位与细胞外DNA（eDNA）在成熟的生物膜，和SslE聚集体也可能与纤维素在某些条件下。我们的研究结果表明，SslE和eDNA之间的相互作用是重要的生物膜成熟在许多大肠杆菌。大肠杆菌菌株和SslE也可能是驱动其他分泌SslE的细菌中生物膜形成的因素。
Escherichia coli is a Gram-negative bacterium that colonises the human intestine and virulent strains can cause severe diarrhoeal and extraintestinal diseases. The protein SslE is secreted by a range of pathogenic and commensal E. coli strains. It can degrade mucins in the intestine, promotes biofilm maturation and it is a major determinant of infection in virulent strains, although how it carries out these functions is not well understood. Here, we examine SslE from the commensal E. coli Waksman and BL21 (DE3) strains and the enterotoxigenic H10407 and enteropathogenic E2348/69 strains. We reveal that SslE has a unique and dynamic structure in solution and in response to acidification within mature biofilms it can form a unique aggregate with amyloid-like properties. Furthermore, we show that both SslE monomers and aggregates bind DNA in vitro and co-localise with extracellular DNA (eDNA) in mature biofilms, and SslE aggregates may also associate with cellulose under certain conditions. Our results suggest that interactions between SslE and eDNA are important for biofilm maturation in many E. coli strains and SslE may also be a factor that drives biofilm formation in other SslE-secreting bacteria.
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影响因子：
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作者：
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