Transcriptome Analysis of mRNA and miRNA in Somatic Embryos of Larix leptolepis Subjected to Hydrogen Treatment.
Transcriptome Analysis of mRNA and miRNA in Somatic Embryos of Larix leptolepis Subjected to Hydrogen Treatment.
复制标题
氢处理落叶松体细胞胚胎 mRNA 和 miRNA 转录组分析
DOI：
10.3390/ijms17111951
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发表时间：
2016-11-22
影响因子：
5.6
通讯作者：
Qi L
中科院分区：
文献类型：
--
作者：
Liu Y;Han S;Ding X;Li X;Zhang L;Li W;Xu H;Li Z;Qi L
氢是一种治疗性抗氧化剂，已在临床试验中广泛使用。它也是一种生物活性分子，可以缓解植物中的非生物胁迫。然而，氢在体细胞胚胎中的生物学效应及其潜在的分子基础在很大程度上仍不清楚。本研究研究了外源H2处理对兴安落叶松体细胞胚胎发生过程中形态和生理的影响。结果表明，暴露在氢气中会增加活跃的前胚胎发生细胞和正常体细胞胚胎的比例。我们对mRNA和microRNA(MiRNA)文库进行了测序，以确定落叶松前胚性团(PEM)在H2处理过程中不同时间点的整体转录组变化。共获得45393个和315个miRNAs。其中，4253个基因和96个miRNAs在氢气处理的文库中与对照相比有差异表达。此外，大量差异表达的mRNAs和miRNAs与ROS的动态平衡和细胞周期调控有关。我们还确定了其中285个miRNAs的4399个潜在靶基因。这里描述的差异表达数据和mRNA-miRNA相互作用网络为决定体细胞胚胎发生过程中氢暴露的PEM性能的分子机制提供了新的见解。
Hydrogen is a therapeutic antioxidant that has been used extensively in clinical trials. It also acts as a bioactive molecule that can alleviate abiotic stress in plants. However, the biological effects of hydrogen in somatic embryos and the underlying molecular basis remain largely unknown. In this study, the morphological and physiological influence of exogenous H2 treatment during somatic embryogenesis was characterized in Larix leptolepis Gordon. The results showed that exposure to hydrogen increased the proportions of active pro-embryogenic cells and normal somatic embryos. We sequenced mRNA and microRNA (miRNA) libraries to identify global transcriptome changes at different time points during H2 treatment of larch pro-embryogenic masses (PEMs). A total of 45,393 mRNAs and 315 miRNAs were obtained. Among them, 4253 genes and 96 miRNAs were differentially expressed in the hydrogen-treated libraries compared with the control. Further, a large number of the differentially expressed mRNAs and miRNAs were related to reactive oxygen species (ROS) homeostasis and cell cycle regulation. We also identified 4399 potential target genes for 285 of the miRNAs. The differential expression data and the mRNA-miRNA interaction network described here provide new insights into the molecular mechanisms that determine the performance of PEMs exposed to H2 during somatic embryogenesis.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
2.9
作者：
Bashandy, Talaat;Meyer, Yves;Reichheld, Jean-Philippe
通讯作者：
Reichheld, Jean-Philippe
DOI：
10.1016/j.bbrc.2008.05.165
发表时间：
2008-08-15
影响因子：
3.1
作者：
Hayashida, Kentaro;Sano, Motoaki;Fukuda, Keiichi
通讯作者：
Fukuda, Keiichi
DOI：
10.1016/j.bbrc.2009.09.047
发表时间：
2009-11-27
影响因子：
3.1
作者：
Itoh, Tomohiro;Fujita, Yasunori;Ito, Masafumi
通讯作者：
Ito, Masafumi
DOI：
10.1016/j.bbrc.2007.07.088
发表时间：
2007-09-28
影响因子：
3.1
作者：
Fukuda, Kei-Ichi;Asoh, Sadamitsu;Ohta, Shigeo
通讯作者：
Ohta, Shigeo