Transcriptome characterization by RNA-seq unravels the mechanisms of butyrate-induced epigenomic regulation in bovine cells.
Transcriptome characterization by RNA-seq unravels the mechanisms of butyrate-induced epigenomic regulation in bovine cells.
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DOI：
10.1371/journal.pone.0036940
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Li CJ
中科院分区：
文献类型：
--
作者：
Wu S;Li RW;Li W;Li CJ
短链脂肪酸(SCFA)，尤其是丁酸，影响细胞的分化、增殖和运动。丁酸盐还通过抑制组蛋白脱乙酰酶(HDACs)诱导细胞周期停滞和凋亡。此外，丁酸盐是细胞中组蛋白超乙酰化的有效诱导剂。因此，SCFA为研究组蛋白乙酰化诱导基因表达的表观基因组调控提供了一个很好的体外模型。在本研究中，我们利用深RNA测序技术(RNA-seq)分析了丁酸诱导牛上皮细胞基因表达的差异。每个样本产生了从57,303,693到78,933,744个不等的读取序列数量。大约11,408个基因受到丁酸盐的显著影响，错误发现率(FDR)为0.05。丁酸盐影响的主要细胞过程包括细胞形态变化、细胞周期停滞和细胞凋亡。我们的结果为深入了解丁酸诱导的转录组改变提供了帮助，这无疑将有助于我们理解丁酸诱导牛细胞表观基因组调控的分子机制。
Short-chain fatty acids (SCFAs), especially butyrate, affect cell differentiation, proliferation, and motility. Butyrate also induces cell cycle arrest and apoptosis through its inhibition of histone deacetylases (HDACs). In addition, butyrate is a potent inducer of histone hyper-acetylation in cells. Therefore, this SCFA provides an excellent in vitro model for studying the epigenomic regulation of gene expression induced by histone acetylation. In this study, we analyzed the differential in vitro expression of genes induced by butyrate in bovine epithelial cells by using deep RNA-sequencing technology (RNA-seq). The number of sequences read, ranging from 57,303,693 to 78,933,744, were generated per sample. Approximately 11,408 genes were significantly impacted by butyrate, with a false discovery rate (FDR) <0.05. The predominant cellular processes affected by butyrate included cell morphological changes, cell cycle arrest, and apoptosis. Our results provided insight into the transcriptome alterations induced by butyrate, which will undoubtedly facilitate our understanding of the molecular mechanisms underlying butyrate-induced epigenomic regulation in bovine cells.
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影响因子：
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DOI：
10.1007/s00432-006-0149-x
发表时间：
2007-02-01
影响因子：
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作者：
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