Peripheral formalin injection induces unique spinal cord microglial phenotypic changes.
Peripheral formalin injection induces unique spinal cord microglial phenotypic changes.
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DOI：
10.1016/j.neulet.2008.11.009
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发表时间：
2009-01-16
影响因子：
2.5
通讯作者：
Mao J
中科院分区：
文献类型：
--
作者：
Fu KY;Tan YH;Sung B;Mao J
小胶质细胞是驻留于脑内的免疫细胞，可被周围组织损伤所激活。在本研究中，我们观察了大鼠后爪注射福尔马林后，脊髓内几种小胶质细胞表面免疫分子的可能诱导，包括白细胞共同抗原(LCA/CD45)、MHC-I类抗原、MHC-II类抗原、Fc受体和CD11c。活化的小胶质细胞中CD45和MHC-I类分子表达上调，在注射福尔马林后第3天表达明显，第7天表达最强。MHCⅡ类分子、CD11c和Fc受体的基础表达水平很低，注射福尔马林后无明显变化。这些结果首次表明，外周注射福尔马林可引起小胶质细胞表型改变，CD45和MHC-I类抗原明显上调。这些数据表明，激活的小胶质细胞的表型变化可能是外周组织损伤后中枢变化的一种独特模式。
Microglia are resident immune cells of brain and activated by peripheral tissue injury. In the present study, we investigated the possible induction of several microglial surface immunomolecules in the spinal cord, including leukocyte common antigen (LCA/CD45), MHC class I antigen, MHC class II antigen, Fc receptor, and CD11c following formalin injection into the rat’s hind paw. CD45 and MHC class I were upregulated in the activated microglia, which was evident on day 3 with the peak expression on day 7 following peripheral formalin injection. There was a very low basal expression of MHC class II, CD11c, and the Fc receptor, which did not change after the formalin injection. These results, for the first time, indicate that peripheral formalin injection can induce phenotypic changes of microglia with distinct upregulation of CD45 and MHC class I antigen. The data suggest that phenotypic changes of the activated microglia may be a unique pattern of central changes following peripheral tissue injury.
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