Convective tissue movements play a major role in avian endocardial morphogenesis.
Convective tissue movements play a major role in avian endocardial morphogenesis.
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DOI：
10.1016/j.ydbio.2011.12.036
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发表时间：
2012-03-15
影响因子：
2.7
通讯作者：
Rongish BJ
中科院分区：
文献类型：
--
作者：
Aleksandrova A;Czirók A;Szabó A;Filla MB;Hossain MJ;Whelan PF;Lansford R;Rongish BJ
内皮细胞在心脏发育和功能中起关键作用，形成早期（管状）心脏的最内层，通过细胞外基质（ECM）与心肌分离。然而，知识是有限的，关于心脏祖细胞和周围ECM的相互作用，在戏剧性的组织重排和伴随的细胞重新定位事件的基础内皮细胞形态发生。通过分析鹌鹑胚胎发育延时记录中免疫标记的ECM成分（纤连蛋白、原纤蛋白-2）和TIE 1阳性心内膜祖细胞的运动，我们证明了前侧板中胚层（LPM）内的初级心脏区域的转化为管状心脏涉及原始心内膜细胞与周围ECM的精确共同运动。因此，管状心脏的ECM包含在早期发育阶段与前LPM相关的细丝。此外，内皮细胞表现出令人惊讶的小定向主动运动，即相对于周围ECM微环境的持续定向运动。这些发现指出了大规模组织运动的重要性，在心脏器官发生过程中，组织运动将细胞对流到适当的位置。
Endocardial cells play a critical role in cardiac development and function, forming the innermost layer of the early (tubular) heart, separated from the myocardium by extracellular matrix (ECM). However, knowledge is limited regarding the interactions of cardiac progenitors and surrounding ECM during dramatic tissue rearrangements and concomitant cellular repositioning events that underlie endocardial morphogenesis. By analyzing the movements of immunolabeled ECM components (fibronectin, fibrillin-2) and TIE1 positive endocardial progenitors in time-lapse recordings of quail embryonic development, we demonstrate that the transformation of the primary heart field within the anterior lateral plate mesoderm (LPM) into a tubular heart involves the precise co-movement of primordial endocardial cells with the surrounding ECM. Thus, the ECM of the tubular heart contains filaments that were associated with the anterior LPM at earlier developmental stages. Moreover, endocardial cells exhibit surprisingly little directed active motility, that is, sustained directed movements relative to the surrounding ECM microenvironment. These findings point to the importance of large-scale tissue movements that convect cells to the appropriate positions during cardiac organogenesis.
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