Optimal conditions for graft survival and reinnervation of denervated muscles after embryonic motoneuron transplantation into peripheral nerves undergoing Wallerian degeneration
Optimal conditions for graft survival and reinnervation of denervated muscles after embryonic motoneuron transplantation into peripheral nerves undergoing Wallerian degeneration
复制标题
胚胎运动神经元移植到经历沃勒变性的周围神经后移植物存活和失神经肌肉神经支配的最佳条件
DOI：
10.1002/term.3223
复制
发表时间：
2021
影响因子：
3.3
通讯作者：
Hirata Hitoshi
中科院分区：
文献类型：
--
作者：
Sawada Hideyoshi;Kurimoto Shigeru;Tokutake Katsuhiro;Saeki Sota;Hirata Hitoshi
将运动神经元移植到发生沃勒变性的周围神经中可能在治疗引起肌肉麻痹的疾病中具有应用。我们研究了失神经肌肉的功能性再支配是否可以通过失神经后的早期或延迟移植来实现。将成年大鼠分为六组，去神经时间逐渐增加（0、1、4、8、12和24周），然后将含有（移植组）或缺乏（手术对照组）解离胚胎运动神经元的培养基接种到腓神经中。移植后3个月进行电生理和组织分析。与手术对照组相比，移植组失神经肌肉的神经再支配显著增加了失神经肌肉的相对肌肉重量，持续失神经1周（分别为0.042% ± 0.0031%和0.032% ± 0.0020%;p= 0.009），4周（分别为0.044% ± 0.0069% vs. 0.026% ± 0.0045%;p= 0.0023）和8周（分别为0.044% ± 0.0029% vs. 0.026% ± 0.0008%;p= 0.0023）。在0、1、4、8和12周移植组中，再神经化肌肉收缩力与幼稚肌肉的比率分别为3.79%、18.99%、8.05%、6.30%和5.80%，表明这些力足以行走。运动神经元植入周围神经的最佳时间为神经切断后1周。但失神经支配24周后移植的神经元存活并再生轴突。这些结果表明，有时间为再生医学中的移植准备细胞，并表明我们的方法可能对目前治疗预计无法恢复的瘫痪肌肉有用。
Motoneuron transplantation into peripheral nerves undergoing Wallerian degeneration may have applications in treating diseases causing muscle paralysis. We investigated whether functional reinnervation of denervated muscle could be achieved by early or delayed transplantation after denervation. Adult rats were assigned to six groups with increasing denervation periods (0, 1, 4, 8, 12, and 24 weeks) before inoculation with culture medium containing (transplantation group) or lacking (surgical control group) dissociated embryonic motoneurons into the peroneal nerve. Electrophysiological and tissue analyses were performed 3 months after transplantation. Reinnervation of denervated muscles significantly increased relative muscle weight in the transplantation group compared with the surgical control group for denervation periods of 1 week (0.042% ± 0.0031% vs. 0.032% ± 0.0020%, respectively;p= 0.009), 4 weeks (0.044% ± 0.0069% vs. 0.026% ± 0.0045%, respectively;p= 0.0023), and 8 weeks (0.044% ± 0.0029% vs. 0.026% ± 0.0008%, respectively;p= 0.0023). The ratios of reinnervated muscle contractile forces to naïve muscle in the 0, 1, 4, 8, and 12 weeks transplantation groups were 3.79%, 18.99%, 8.05%, 6.30%, and 5.80%, respectively, indicating that these forces were sufficient for walking. The optimal implantation time for transplantation of motoneurons into the peripheral nerve was 1 week after nerve transection. However, the neurons transplanted 24 weeks after denervation survived and regenerated axons. These results indicated that there is time for preparing cells for transplantation in regenerative medicine and suggested that our method may be useful for paralysed muscles that are not expected to recover with current treatment.
登录
查看更多内容
周围神经的胚胎索移植可恢复骨骼肌功能。
影响因子：
2.5
作者：
Thomas,CK;Erb,DE;Grumbles,RM;Bunge,RP
通讯作者：
Bunge,RP
影响因子：
2.5
作者：
Botterman,BR;Iwamoto,GA;Gonyea,WJ
通讯作者：
Gonyea,WJ
运动神经元成功移植到周围神经取决于移植细胞的数量
影响因子：
0.9
作者：
Shuichi Kato;S. Kurimoto;Tomonori Nakano;H. Yoneda;H. Ishii;Satoka MITA;H. Hirata
通讯作者：
H. Hirata
影响因子：
3.3
作者：
Klein, Cliff S.;Peterson, Lillian B.;Thomas, Christine K.
通讯作者：
Thomas, Christine K.
运动单位估计：焦虑和成就
影响因子：
3.4
作者：
A. McComas
通讯作者：
A. McComas