Horizontal Transfer of Different erm(B)-Carrying Mobile Elements Among Streptococcus suis Strains With Different Serotypes.
Horizontal Transfer of Different erm(B)-Carrying Mobile Elements Among Streptococcus suis Strains With Different Serotypes.
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不同血清型猪链球菌菌株中携带不同erm(B)移动元件的水平转移
DOI：
10.3389/fmicb.2021.628740
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发表时间：
2021
影响因子：
5.2
通讯作者：
Wang L
中科院分区：
文献类型：
--
作者：
Chen L;Huang J;Huang X;He Y;Sun J;Dai X;Wang X;Shafiq M;Wang L
大环内酯类耐药猪链球菌在世界范围内高度流行。由移动的遗传元件（MGE）特别是整合和接合元件（ICE）介导的β-内酰胺酶（B）基因的获得被认为是大环内酯类抗生素耐药链球菌菌株快速传播的主要原因。然而，关于导致大环内酯类耐药性广泛存在的不同的β（B）携带元件及其在S.对suis仍然知之甚少。在本研究中，两个携带有β（B）和泰特（O）的推定ICE，命名为ICESsuYSB 17_rplL和ICESsuYSJ15_rplL，以及一个新的携带有β（B）和aadE-spw-like的基因组岛（GI），命名为GISsuJHJ 17_rpsI，被从染色体上切下并在S.不同血清型的猪群。ICESsuYSB 17_rplL和ICESsuYSJ15_rplL整合在rplL基因下游，rplL基因是ICESa 2603家族的保守基因座。将不具有属于接合模块的基因的GISsuJHJ17_rpsI整合到rpsI位点。通过生长曲线和竞争试验，所有接合子与受体相比，没有表现出明显的适应度代价。结果表明，不同的β（B）-携带的元素，并强调这些元素在传播的大环内酯类耐药的S。猪。
Macrolide-resistant Streptococcus suis is highly prevalent worldwide. The acquisition of the erm(B) gene mediated by mobile genetic elements (MGEs) in particular integrative and conjugative elements (ICEs) is recognized as the main reason for the rapid spread of macrolide-resistant streptococcal strains. However, knowledge about different erm(B)-carrying elements responsible for the widespread of macrolide resistance and their transferability in S. suis remains poorly understood. In the present study, two erm(B)- and tet(O)-harboring putative ICEs, designated as ICESsuYSB17_rplL and ICESsuYSJ15_rplL, and a novel erm(B)- and aadE-spw-like-carrying genomic island (GI), named GISsuJHJ17_rpsI, were identified to be excised from the chromosome and transferred among S. suis strains with different serotypes. ICESsuYSB17_rplL and ICESsuYSJ15_rplL were integrated downstream the rplL gene, a conserve locus of the ICESa2603 family. GISsuJHJ17_rpsI, with no genes belonging to the conjugation module, was integrated into the site of rpsI. All transconjugants did not exhibit obvious fitness cost by growth curve and competition assays when compared with the recipient. The results demonstrate that different erm(B)-carrying elements were presented and highlight the role of these elements in the dissemination of macrolide resistance in S. suis.
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影响因子：
11.8
作者：
Vela AI;Villalón P;Sáez-Nieto JA;Chacón G;Domínguez L;Fernández-Garayzábal JF
通讯作者：
Fernández-Garayzábal JF
影响因子：
3.6
作者：
Daccord, Aurelie;Ceccarelli, Daniela;Burrus, Vincent
通讯作者：
Burrus, Vincent
影响因子：
64.8
作者：
Beaber, JW;Hochhut, B;Waldor, MK
通讯作者：
Waldor, MK
影响因子：
3.7
作者：
Chen C;Tang J;Dong W;Wang C;Feng Y;Wang J;Zheng F;Pan X;Liu D;Li M;Song Y;Zhu X;Sun H;Feng T;Guo Z;Ju A;Ge J;Dong Y;Sun W;Jiang Y;Wang J;Yan J;Yang H;Wang X;Gao GF;Yang R;Wang J;Yu J
通讯作者：
Yu J
DOI：
10.1007/s10096-016-2616-x
发表时间：
2016-06
期刊：
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
影响因子：
--
作者：
Bojarska A;Molska E;Janas K;Skoczyńska A;Stefaniuk E;Hryniewicz W;Sadowy E
通讯作者：
Sadowy E