The Effect of Sulforaphane on Glyoxalase I Expression and Activity in Peripheral Blood Mononuclear Cells.
The Effect of Sulforaphane on Glyoxalase I Expression and Activity in Peripheral Blood Mononuclear Cells.
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DOI：
10.3390/nu10111773
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发表时间：
2018-11-15
期刊：
影响因子：
5.9
通讯作者：
Oliviero T
中科院分区：
文献类型：
--
作者：
Alfarano M;Pastore D;Fogliano V;Schalkwijk CG;Oliviero T
研究表明，萝卜硫素（SR）的潜在健康有益作用是西兰花中形成的一种化合物，是许多机制的结果，包括上调第二阶段解毒酶。最近的研究表明，SR增加了glycoprotein酶1（Glo 1）的表达/活性，glycoprotein酶1是一种参与甲基乙二醛降解的酶，是晚期糖基化终产物的主要前体。这些化合物与糖尿病并发症和其他与年龄有关的疾病有关。在这项研究中，从8名健康志愿者的外周血单个核细胞（PBMC）中Glo 1的表达/活性的SR的影响进行了研究。分离PBMC并与SR（通过食用花椰菜部分可达到2.5 μ M浓度）孵育24 h和48 h。Glo 1活性/表达，还原型谷胱甘肽（GSH）和谷胱甘肽-S-转移酶基因表达进行了测定。Glo 1活性不受影响，而在48小时后，观察到其基因表达轻微但显著增加（1.03倍）。孵育24小时后，GSTP 1表达略有增加（1.08倍），而亚型GSTT 2和GSTM 2的表达低于检测限。GSH急剧下降，表明GSH-SR加合物的形成可能会影响SR的可用性。这些结果表明，通过西兰花消费或SR补充剂定期暴露于SR可能会增强Glo 1。
Studies demonstrate that the potential health-beneficial effect of sulforaphane (SR), a compound formed in broccoli, is the result of a number of mechanisms including upregulation of phase two detoxification enzymes. Recent studies suggest that SR increases expression/activity of glyoxalase 1 (Glo1), an enzyme involved in the degradation of methylglyoxal, is major precursor of advanced glycation end products. Those compounds are associated with diabetes complications and other age-related diseases. In this study, the effect of SR on the expression/activity of Glo1 in peripheral blood mononuclear cells (PBMCs) from 8 healthy volunteers was investigated. PBMCs were isolated and incubated with SR (2.5 μM-concentration achievable by consuming a broccoli portion) for 24 h and 48 h. Glo1 activity/expression, reduced glutathione (GSH), and glutathione-S-transferase gene expression were measured. Glo1 activity was not affected while after 48 h a slight but significant increase of its gene expression (1.03-fold) was observed. GSTP1 expression slightly increased after 24 h incubation (1.08-fold) while the expressions of isoform GSTT2 and GSTM2 were below the limit of detection. GSH sharply decreased, suggesting the formation of GSH-SR adducts that may have an impact SR availability. Those results suggest that a regular exposure to SR by broccoli consumption or SR supplements may enhance Glo1.
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影响因子：
6.1
作者：
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通讯作者：
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影响因子：
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作者：
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