Identification of a Wheat-Psathyrostachys huashanica 7Ns Ditelosomic Addition Line Conferring Early Maturation by Cytological Analysis and Newly Developed Molecular and FISH Markers.
Identification of a Wheat-Psathyrostachys huashanica 7Ns Ditelosomic Addition Line Conferring Early Maturation by Cytological Analysis and Newly Developed Molecular and FISH Markers.
复制标题
DOI：
10.3389/fpls.2021.784001
复制
发表时间：
2021
影响因子：
5.6
通讯作者：
Kang H
中科院分区：
文献类型：
--
作者：
Tan B;Zhao L;Li L;Zhang H;Zhu W;Xu L;Wang Y;Zeng J;Fan X;Sha L;Wu D;Cheng Y;Zhang H;Chen G;Zhou Y;Kang H
小麦早熟是小麦育种的重要目标之一，它有利于多熟种植，减少灾害和病害损失，保证小麦稳产，提高经济效益。从小麦近缘野生种中发掘新种质是小麦早熟育种的有效手段。华山新麦草不包括中国。KUO（2n=2x=14，NsNs）是小麦遗传改良有用基因的一个有希望的来源。本研究以普通小麦与华山松远缘杂交获得的新小麦-华山松品系DT 23为材料。荧光原位杂交（FISH）和序列基因组原位杂交（GISH）分析表明，DT 23是一个稳定的小麦-华山松双端体异附加系。FISH和基于PCR的landmark独特基因标记分析进一步揭示了DT 23是小麦-华山松7 Ns双端体异附加系。幼穗分化和生育期的观察表明，DT 23比亲本早熟。这是首次报道的与华山松第7组染色体相关的早熟性基因。利用特异位点扩增片段测序技术，开发了华山松7 Ns染色体的45个新的特异分子标记和19个特异FISH探针。分子标记验证分析表明，两个特异性标记区分了华山松的Ns基因组染色体和其他小麦近缘种的染色体。这些新开发的FISH探针特异性地检测到小麦背景下华山松的Ns基因组染色体。DT 23系在早熟小麦育种中具有重要的应用价值。本研究开发的特异性标记和FISH探针可用于华山松不同材料中携带优良基因的染色体和染色体片段的检测和追踪。
Early maturation is an important objective in wheat breeding programs that could facilitate multiple-cropping systems, decrease disaster- and disease-related losses, ensure stable wheat production, and increase economic benefits. Exploitation of novel germplasm from wild relatives of wheat is an effective means of breeding for early maturity. Psathyrostachys huashanica Keng f. ex P. C. KUO (2n=2x=14, NsNs) is a promising source of useful genes for wheat genetic improvement. In this study, we characterized a novel wheat-P. huashanica line, DT23, derived from distant hybridization between common wheat and P. huashanica. Fluorescence in situ hybridization (FISH) and sequential genomic in situ hybridization (GISH) analyses indicated that DT23 is a stable wheat-P. huashanica ditelosomic addition line. FISH painting and PCR-based landmark unique gene markers analyses further revealed that DT23 is a wheat-P. huashanica 7Ns ditelosomic addition line. Observation of spike differentiation and the growth period revealed that DT23 exhibited earlier maturation than the wheat parents. This is the first report of new earliness per se (Eps) gene(s) probably associated with a group 7 chromosome of P. huashanica. Based on specific locus-amplified fragment sequencing technology, 45 new specific molecular markers and 19 specific FISH probes were developed for the P. huashanica 7Ns chromosome. Marker validation analyses revealed that two specific markers distinguished the Ns genome chromosomes of P. huashanica and the chromosomes of other wheat-related species. These newly developed FISH probes specifically detected Ns genome chromosomes of P. huashanica in the wheat background. The DT23 line will be useful for breeding early maturing wheat. The specific markers and FISH probes developed in this study can be used to detect and trace P. huashanica chromosomes and chromosomal segments carrying elite genes in diverse materials.
登录
查看更多内容
影响因子：
3.1
作者：
Fedak, G
通讯作者：
Fedak, G
影响因子：
0.9
作者：
BADEN, C
通讯作者：
BADEN, C
影响因子：
3.1
作者：
Du, Wanli;Wang, Jing;Chen, Xinhong
通讯作者：
Chen, Xinhong
影响因子：
3.1
作者：
Cseh, A.;Kruppa, K.;Molnar-Lang, M.
通讯作者：
Molnar-Lang, M.
影响因子：
1.9
作者：
Du, Wanli;Wang, Jing;Chen, Xinhong
通讯作者：
Chen, Xinhong