Enzymatic formation of apo-carotenoids from the xanthophyll carotenoids lutein, zeaxanthin and β-cryptoxanthin by ferret carotene-9',10'-monooxygenase.
Enzymatic formation of apo-carotenoids from the xanthophyll carotenoids lutein, zeaxanthin and β-cryptoxanthin by ferret carotene-9',10'-monooxygenase.
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DOI：
10.1016/j.abb.2010.11.005
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发表时间：
2011-02-01
影响因子：
3.9
通讯作者：
Wang XD
中科院分区：
文献类型：
--
作者：
Mein JR;Dolnikowski GG;Ernst H;Russell RM;Wang XD
叶黄素类胡萝卜素，如叶黄素、玉米黄质和β-隐黄质，可能对慢性和退行性疾病提供潜在的健康益处。研究叶黄素代谢途径对了解其生物学功能具有重要意义。胡萝卜素-15,15 ' -单加氧酶（CMO1）已被证明参与维生素A的形成，而最近的研究表明，胡萝卜素-9 ',10 ' -单加氧酶（CMO2）可能比以前认识到的具有更广泛的底物特异性。在这项体外研究中，我们研究了杆状病毒产生的重组雪貂CMO2对类胡萝卜素底物玉米黄质、叶黄素和β-隐黄质的裂解活性。利用HPLC、LC-MS和GC-MS，我们鉴定了挥发性和非挥发性类伪胡萝卜素产品，包括3-OH-β-ionone、3-OH-α-ionone、β-ionone、3-OH-α-apo-10 ' -胡萝卜素、3-OH-β-apo-10 ' -胡萝卜素和β-apo-10 ' -胡萝卜素，表明9,10和9 ‘,10 ’碳-碳双键均被裂解。酶动力学分析表明，叶黄素和玉米黄质比β-隐黄质更容易裂解，表明CMO2在非维生素a原类胡萝卜素代谢中起关键作用。此外，3-OH-β-apo-10 ' -胡萝卜素与CMO2裂解物孵育可形成3-OH-β-离子酮。在NAD+存在下，3-OH-β-apo-10′-胡萝卜素与雪貂肝脏匀浆体外孵育形成3-OH-β-apo-10′-胡萝卜素酸。由于类载胡萝卜素在多种生物过程中发挥着重要的信号分子作用，哺乳动物CMO2酶切黄叶素代表了脊椎动物类胡萝卜素代谢和生物学功能研究的新途径。
Xanthophyll carotenoids, such as lutein, zeaxanthin and β-cryptoxanthin, may provide potential health benefits against chronic and degenerative diseases. Investigating pathways of xanthophyll metabolism are important to understanding their biological functions. Carotene-15,15’-monooxygenase (CMO1) has been shown to be involved in vitamin A formation, while recent studies suggest that carotene-9’,10’-monooxygenase (CMO2) may have a broader substrate specificity than previously recognized. In this in vitro study, we investigated baculovirus-generated recombinant ferret CMO2 cleavage activity towards the carotenoid substrates zeaxanthin, lutein and β-cryptoxanthin. Utilizing HPLC, LC-MS and GC-MS, we identified both volatile and non-volatile apocarotenoid products including 3-OH-β-ionone, 3-OH-α-ionone, β-ionone, 3-OH-α-apo-10’-carotenal, 3-OH-β-apo-10’-carotenal, and β-apo-10’-carotenal, indicating cleavage at both the 9,10 and 9’,10’ carbon-carbon double bond. Enzyme kinetic analysis indicated the xanthophylls zeaxanthin and lutein are preferentially cleaved over β-cryptoxanthin, indicating a key role of CMO2 in non-provitamin A carotenoid metabolism. Furthermore, incubation of 3-OH-β-apo-10’-carotenal with CMO2 lysate resulted in the formation of 3-OH-β-ionone. In the presence of NAD+, in vitro incubation of 3-OH-β-apo-10’-carotenal with ferret hepatic homogenates formed 3-OH-β-apo-10’-carotenoic acid. Since apo-carotenoids serve as important signaling molecules in a variety of biological processes, enzymatic cleavage of xanthophylls by mammalian CMO2 represents a new avenue of research regarding vertebrate carotenoid metabolism and biological function.
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黄色皮肤基因的鉴定显示了家鸡的杂种起源。
影响因子：
4.5
作者：
Eriksson, Jonas;Larson, Greger;Gunnarsson, Ulrika;Bed'hom, Bertrand;Tixier-Boichard, Michele;Stromstedt, Lina;Wright, Dominic;Jungerius, Annemieke;Vereijken, Addie;Randi, Ettore;Jensen, Per;Andersson, Leif
通讯作者：
Andersson, Leif
DOI：
10.1006/bbrc.1995.2610
发表时间：
1995-11-02
影响因子：
3.1
作者：
JEONG, TC;KIM, HJ;ROH, JK
通讯作者：
ROH, JK
影响因子：
4.8
作者：
Kiefer, C;Hessel, S;von Lintig, J
通讯作者：
von Lintig, J
影响因子：
7.4
作者：
Dachtler, M;Glaser, T;Albert, K
通讯作者：
Albert, K
影响因子：
3.4
作者：
Kalariya, Nilesh M.;Ramana, Kota V.;van Kuijk, Frederik J. G. M.
通讯作者：
van Kuijk, Frederik J. G. M.