3D single cell scale anatomical map of sex-dependent variability of the rat intrinsic cardiac nervous system.
3D single cell scale anatomical map of sex-dependent variability of the rat intrinsic cardiac nervous system.
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DOI：
10.1016/j.isci.2021.102795
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发表时间：
2021-07-23
期刊：
影响因子：
5.8
通讯作者：
Schwaber JS
中科院分区：
文献类型：
--
作者：
Leung C;Robbins S;Moss A;Heal M;Osanlouy M;Christie R;Farahani N;Monteith C;Chen J;Hunter P;Tappan S;Vadigepalli R;Cheng ZJ;Schwaber JS
我们开发并分析了大鼠内在心脏神经系统（ICNS）在四个男性和三个女性心脏的单细胞尺度解剖图。我们发现ICNS在个体之间具有可靠的结构组织计划，这为进一步分析ICNS在心脏功能和疾病中的作用提供了基础。通过三维可视化和数据驱动聚类评价ICNS的分布。ICNS神经元在所有雄性和雌性大鼠心脏的模式，分布和聚类是高度保守的，表现出一致的组织计划，其中不同的神经元簇始终本地化。雌性心脏的神经元较少，堆积密度较低，分布略减少，但定位相同。我们将每个心脏的解剖数据配准到几何支架上，将其3D坐标标准化以实现常见解剖平面的标准化，并提供了一条可以集成和比较多个实验结果和数据类型的路径。大鼠内在心脏神经系统的3D解剖图神经元的优先组织在选择的心脏解剖区域内在心脏神经元的保守的解剖组织跨性别映射高分辨率心脏解剖数据的一般化3D支架性别生物学;心血管医学;成像解剖学;系统神经科学
We developed and analyzed a single cell scale anatomical map of the rat intrinsic cardiac nervous system (ICNS) across four male and three female hearts. We find the ICNS has a reliable structural organizational plan across individuals that provide the foundation for further analyses of the ICNS in cardiac function and disease. The distribution of the ICNS was evaluated by 3D visualization and data-driven clustering. The pattern, distribution, and clustering of ICNS neurons across all male and female rat hearts is highly conserved, demonstrating a coherent organizational plan where distinct clusters of neurons are consistently localized. Female hearts had fewer neurons, lower packing density, and slightly reduced distribution, but with identical localization. We registered the anatomical data from each heart to a geometric scaffold, normalizing their 3D coordinates for standardization of common anatomical planes and providing a path where multiple experimental results and data types can be integrated and compared. 3D anatomical map of rat intrinsic cardiac nervous system Preferential organization of neurons in select cardiac anatomical regions Conserved anatomical organization of intrinsic cardiac neurons across sexes Generalized 3D scaffold for mapping of high-resolution cardiac anatomical data Biology of gender; Cardiovascular medicine; Imaging anatomy; Systems neuroscience
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影响因子：
2.7
作者：
Hardwick, Jean C.;Ryan, Shannon E.;Beaumont, Eric;Ardell, Jeffrey L.;Southerland, E. Marie
通讯作者：
Southerland, E. Marie
影响因子：
3
作者：
FUNKALEA, GD;SCHWABER, JS
通讯作者：
SCHWABER, JS
影响因子：
--
作者：
MIZERES, NJ
通讯作者：
MIZERES, NJ
影响因子：
64.8
作者：
通讯作者：
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影响因子：
2.7
作者：
Li, Liang;Hatcher, Jeffrey T.;Hoover, Donald B.;Gu, He;Wurster, Robert D.;Cheng, Zixi (Jack)
通讯作者：
Cheng, Zixi (Jack)