MicA sRNA links the PhoP regulon to cell envelope stress.
MicA sRNA links the PhoP regulon to cell envelope stress.
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DOI：
10.1111/j.1365-2958.2010.07115.x
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发表时间：
2010-04
影响因子：
3.6
通讯作者：
Guillier M
中科院分区：
文献类型：
--
作者：
Coornaert A;Lu A;Mandin P;Springer M;Gottesman S;Guillier M
细菌中存在许多基因表达的小rna调控因子。它们中的一大类与RNA伴侣Hfq结合，并通过碱基配对与目标mrna相互作用，从而影响它们的翻译和/或稳定性。它们通常有多个直接靶标，其中一些可能是调节因子本身，因此单个sRNA的产生可以影响数十个基因的表达。在本研究中，我们发现大肠杆菌多效PhoPQ双组分体系的合成受到MicA的抑制，MicA是一种依赖σ e的孔蛋白生物形成的sRNA调节剂。MicA在phoP的翻译起始区直接与phoPQ mRNA配对，可能通过与核糖体结合竞争来抑制翻译。因此，MicA下调了PhoPQ调控的几个成员。通过将PhoPQ与σE联系起来，我们的研究结果表明，包膜胁迫会调节主要的细胞过程，如Mg2+运输、毒力、LPS修饰或对抗菌肽的抗性。此外，我们发现Hfq强烈地独立于MicA影响phoP的表达，这提出了更多的sRNAs（仍有待鉴定）可能调节PhoPQ合成的可能性。
Numerous small RNAs regulators of gene expression exist in bacteria. A large class of them binds to the RNA chaperone Hfq and act by base-pairing interactions with their target-mRNA, thereby affecting their translation and/or stability. They often have multiple direct targets, some of which may be regulators themselves, and production of a single sRNA can therefore affect the expression of dozens of genes. We show in this study that the synthesis of the E. coli pleiotropic PhoPQ two-component system is repressed by MicA, a σE-dependent sRNA regulator of porin biogenesis. MicA directly pairs with phoPQ mRNA in the translation initiation region of phoP and presumably inhibits translation by competiting with ribosome binding. Consequently, MicA down-regulates several members of the PhoPQ regulon. By linking PhoPQ to σE, our findings suggest that major cellular processes such as Mg2+ transport, virulence, LPS modification or resistance to antimicrobial peptides are modulated in response to envelope stress. In addition, we found that Hfq strongly affects the expression of phoP independently of MicA, raising the possibility that even more sRNAs, that remain to be identified, could regulate PhoPQ synthesis.
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影响因子：
14.9
作者：
Guillier, Maude;Gottesman, Susan
通讯作者：
Gottesman, Susan
影响因子：
3.2
作者：
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作者：
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DOI：
10.1073/pnas.0700025104
发表时间：
2007-10-09
影响因子：
11.1
作者：
Miyashiro, Tim;Goulian, Mark
通讯作者：
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DOI：
10.1073/pnas.032066599
发表时间：
2002-04-02
影响因子：
11.1
作者：
Massé, E;Gottesman, S
通讯作者：
Gottesman, S