Polymerase chain reaction.
Polymerase chain reaction.
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DOI:
10.1038/jid.2013.1
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发表时间:
2013-03
影响因子:
6.5
通讯作者:
Avashia, Nidhi
中科院分区:
文献类型:
--
作者:
Garibyan, Lilit;Avashia, Nidhi
The advent of the polymerase chain reaction (PCR) radically transformed biological science from the time it was discovered (Mullis, 1990). For the first time, PCR allowed for specific detection and production of large amounts of DNA. PCR-based strategies have propelled vast scientific endeavors such as the Human Genome Project. The technique is widely used by clinicians and researchers to diagnose diseases, clone and sequence genes, and carry out sophisticated quantitative and genomic studies in a rapid and very sensitive manner. One of the most important medical applications of the classic PCR method is detection of pathogens. In addition, the PCR assay is used in forensic medicine to identify criminals. Because of its widespread use, it is important to understand the basic principles of PCR and how its use can be modified to provide for sophisticated analysis of genes and the genome.
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影响因子:
9.3
作者:
Stahlberg, Anders;Thomsen, Christer;Aman, Pierre
通讯作者:
Aman, Pierre
影响因子:
3
作者:
MULLIS, KB
通讯作者:
MULLIS, KB
影响因子:
2.1
作者:
Valasek, MA;Repa, JJ
通讯作者:
Repa, JJ
影响因子:
6.5
作者:
Riedl, E;Tada, Y;Udey, MC
通讯作者:
Udey, MC
DOI:
10.1089/dna.1.1988.7.441
发表时间:
1988-07-01
期刊:
DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY
影响因子:
--
作者:
WEIER, HU;GRAY, JW
通讯作者:
GRAY, JW