A novel role for scavenger receptor B1 as a contributor to the capture of specific volatile odorants in the nasal cavity.
A novel role for scavenger receptor B1 as a contributor to the capture of specific volatile odorants in the nasal cavity.
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清道夫受体 B1 的新作用是捕获鼻腔中特定的挥发性气味物质。
DOI：
10.2220/biomedres.39.117
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Kazuo Inoue
中科院分区：
文献类型：
--
作者：
S. Tsuzuki;Yusaku Kimoto;Shinhye Lee;T. Sugawara;Y. Manabe;Kazuo Inoue
B类清道夫受体，清道夫受体B1（SR-B1）和分化簇36（CD 36），是广泛表达的细胞表面蛋白，并且被认为在哺乳动物的脂质和脂蛋白代谢中起多方面的作用。由于其能够识别不同的气味活性挥发性化合物及其存在于小鼠嗅上皮中，CD 36最近已成为鼻腔内气味检测的参与者。然而，没有人试图评估SR-B1是否具有这种作用。在这项研究中，我们利用受体的肽模拟物进行了无细胞体外测定，并证明SR-B1可以识别脂肪醛（例如，十四醛），一种独特的挥发性气味剂，作为潜在的配体。采用逆转录-聚合酶链反应（RT-PCR）和免疫印迹（Western immunoblot）方法检测SR-B1 mRNA和蛋白在小鼠嗅组织中的表达。最后，我们在体内的嗅觉上皮的表面层，这是气味检测的第一线的SR-B1的分布的化学定位。这些发现揭示了SR-B1作为捕获哺乳动物鼻腔中特定气味的贡献者的新作用。
Class B scavenger receptors, scavenger receptor B1 (SR-B1) and cluster of differentiation 36 (CD36), are broadly expressed cell-surface proteins and are believed to serve as multifaceted players in lipid and lipoprotein metabolism in mammals. Because of its ability to recognise distinct odour-active volatile compounds and its presence in murine olfactory epithelium, CD36 has recently emerged as a participant in the detection of odorants within the nasal cavity. However, there have been no attempts to assess whether SR-B1 has such a role. In this study, we performed a cell-free in-vitro assay utilising a peptide mimic of the receptor, and demonstrated that SR-B1 could recognise aliphatic aldehydes (e.g., tetradecanal), a distinct class of volatile odorants, as potential ligands. By reverse transcription-polymerase chain reaction and western immunoblot analyses, we detected the expression of SR-B1 mRNA and protein, respectively, in mouse olfactory tissue. Finally, we immunohistochemically mapped the distribution of SR-B1 in the surface layer of olfactory epithelium in vivo, which is the first line of odorant detection. These findings uncover a novel role for SR-B1 as a contributor to the capture of specific odorants in the nasal cavity of mammals.
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影响因子：
6.5
作者：
K. Gillotte-Taylor;A. Boullier;J. L. Witztum;D. Steinberg;O. Quehenberger
通讯作者：
K. Gillotte-Taylor;A. Boullier;J. L. Witztum;D. Steinberg;O. Quehenberger
DOI：
10.14800/rci.811
发表时间：
2015
期刊：
Receptors & clinical investigation
影响因子：
--
作者：
Shannahan JH;Bai W;Brown JM
通讯作者：
Brown JM
DOI：
10.1016/s0021-9258(17)31921-x
发表时间：
1994-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
S. Acton;Philipp E. Scherer;H. Lodish;M. Krieger
通讯作者：
S. Acton;Philipp E. Scherer;H. Lodish;M. Krieger
CD36参与了鼠嗅觉系统的油酸检测。
影响因子：
5.3
作者：
Oberland S;Ackels T;Gaab S;Pelz T;Spehr J;Spehr M;Neuhaus EM
通讯作者：
Neuhaus EM