Characterization of a new model of thromboembolic stroke in C57 black/6J mice.
Characterization of a new model of thromboembolic stroke in C57 black/6J mice.
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DOI：
10.1007/s12975-013-0315-9
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发表时间：
2014-08
影响因子：
6.9
通讯作者：
Meairs, Stephen
中科院分区：
文献类型：
--
作者：
Ansar, Saema;Chatzikonstantinou, Eva;Wistuba-Schier, Anja;Mirau-Weber, Silvia;Fatar, Marc;Hennerici, Michael G.;Meairs, Stephen
本研究描述了 C57 black/6J 小鼠大脑中动脉血栓栓塞性中风的新模型，从而为利用该模型研究转基因小鼠的缺血性中风提供了机会。通过将 1.5 或 3.0 UI 凝血酶直接局部注射到 C57 black/6J 小鼠的右侧 MCA 中诱导血栓栓塞性中风。通过激光多普勒血流计连续测量脑血流 (CBF) 速度，从而记录 MCA 闭塞和自发再通情况。 24小时后，所有动物均被安乐死。以 400 μm 的间隔切割冰冻切片，并用高对比度方法进行银染色，用于梗塞大小的体积评估。通过免疫荧光法研究白细胞介素 (IL)-6、肿瘤坏死因子-α (TNF-α)、caspase-3 和 hsp 70 蛋白水平。凝血酶注射导致所有动物形成凝块。 63% 的小鼠发生皮质梗塞，而 37% 的小鼠在注射凝血酶后的前 20 分钟内出现自发的 MCA 再通。在成功 MCA 闭塞并随后发生梗塞的情况下，凝块在形成后 2 小时内保持稳定。随后，20% 的患者自发再通。梗塞仅限于皮质，1.5 UI 凝血酶的平均病变体积为 36±5，3.0 UI 凝血酶的平均病变体积为 56±±8。 MCAO 后 IL-6、TNF-α、caspase-3 和 hsp 70 的蛋白水平显着升高。结果表明，小鼠血栓栓塞性中风模型在 C57 black/6J 小鼠中产生大小一致的皮质梗塞，这取决于用于凝块形成的凝血酶的量。 20% 的小鼠在缺血 2 小时后会发生自发的 MCA 再通。因此，血栓栓塞模型是适用于 C57 black/6J 小鼠的中风模型，它模仿了人类中风的许多特征，包括自发再通。然而，使用该模型时必须考虑 Swiss 小鼠和 C57 black/6J 小鼠之间的品系差异。
This study characterizes a new model of thromboembolic stroke of the middle cerebral artery in C57 black/6J mice, thus offering an opportunity to use the model for studying ischemic stroke in transgenic mice. Thromboembolic stroke was induced by local injection of either 1.5 or 3.0 UI of thrombin directly into the right MCA of C57 black/6J mice. Cerebral blood flow (CBF) velocity was measured continuously by laser Doppler flowmetry, which allowed documentation of both MCA occlusion and of spontaneous recanalization. After 24 h, all animals were euthanized. Cryosections were cut at 400-μm intervals and silver stained with the high-contrast method for volumetric assessment of infarct size. Interleukin (IL)-6, tumor necrosis factor-alpha (TNF-α), caspase-3 and hsp 70 protein levels were investigated by immunofluorescence. Thrombin injection resulted in clot formation in all animals. Cortical infarction occurred in 63 % of the mice while 37 % had a spontaneous MCA recanalization during the first 20 min following thrombin injection. In cases of successful MCA occlusion with consequent infarction, the clot was stable up to 2 h after formation. Subsequently, 20 % recanalized spontaneously. Infarctions were restricted to the cortex with a mean lesion volume of 36 ± 5 for 1.5 UI and 56 ± 8 for 3.0 UI thrombin. Protein levels of IL-6, TNF-α, caspase-3, and hsp 70 were significantly increased after MCAO. The results demonstrate that the mouse thromboembolic stroke model produces cortical infarctions of consistent size in C57 black/6J mice, which is dependent upon the amount of thrombin used for clot formation. Spontaneous MCA recanalization occurs after 2 h of ischemia in 20 % of mice. Thus, the thromboembolic model is an applicable stroke model for C57 black/6J mice, which mimics many of the features of human stroke, including spontaneous recanalization. However, strain differences between Swiss and C57 black/6J mice must be taken into account when using the model.
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影响因子：
6.3
作者：
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通讯作者：
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