RNA-Seq analysis reveals insight into enhanced rice Xa7-mediated bacterial blight resistance at high temperature.
RNA-Seq analysis reveals insight into enhanced rice Xa7-mediated bacterial blight resistance at high temperature.
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RNA-Seq 分析揭示了增强水稻 Xa7 介导的高温白叶枯病抗性的见解
DOI：
10.1371/journal.pone.0187625
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Leach JE
中科院分区：
文献类型：
--
作者：
Cohen SP;Liu H;Argueso CT;Pereira A;Vera Cruz C;Verdier V;Leach JE
植物病害是全球农业面临的重大挑战，非生物环境因素加剧了植物病害的发生。在一些植物与病原体相互作用过程中，热胁迫使病原体能够克服宿主的抗性，考虑到与气候变化相关的全球变暖趋势，这种现象可能严重影响作物生产力。尽管这一现象很重要，但人们对其潜在的分子机制知之甚少。为了更好地了解寄主植物在高温和病原体同时胁迫下的反应，我们对含有抗白叶枯病(R)基因Xa7的水稻品种IRBB61进行了转录组学实验，这些水稻品种在高温胁迫下感染了水稻白叶枯病病菌Xanthomonas oryzae。相对于其他R基因介导的抗性，xa7介导的抗性是不寻常的，因为它在高温下功能更好。利用RNA-Seq技术，我们在水稻品种IRBB61中鉴定出8499个差异表达的温度响应基因，在三个时间点上经历了易感和抗性相互作用。值得注意的是，植物激素脱落酸生物合成和应答通路的基因在模拟处理和易感相互作用的植物中都受到高温的上调，而在表现xa7介导抗性的植物中受到高温的抑制。水杨酸是一种重要的植物抗病性激素，在高温作用下，对水杨酸的应答基因在易感和抗性相互作用中均被下调，这表明xa7介导的高温抗性增强并不依赖于水杨酸信号。在基因上游的启动子区域发现了一个类似于已知脱落酸反应的顺式调控元件的DNA序列基序，该基因在易感相互作用中上调，而在抗性相互作用中下调。我们的研究结果表明，植物激素脱落酸是植物高温胁迫和病原体攻击转录反应通路相互作用的重要节点。这一途径中的基因代表了未来研究的重要焦点，以确定植物如何进化以应对同时发生的非生物和生物胁迫。
Plant disease is a major challenge to agriculture worldwide, and it is exacerbated by abiotic environmental factors. During some plant-pathogen interactions, heat stress allows pathogens to overcome host resistance, a phenomenon which could severely impact crop productivity considering the global warming trends associated with climate change. Despite the importance of this phenomenon, little is known about the underlying molecular mechanisms. To better understand host plant responses during simultaneous heat and pathogen stress, we conducted a transcriptomics experiment for rice plants (cultivar IRBB61) containing Xa7, a bacterial blight disease resistance (R) gene, that were infected with Xanthomonas oryzae, the bacterial blight pathogen of rice, during high temperature stress. Xa7-mediated resistance is unusual relative to resistance mediated by other R genes in that it functions better at high temperatures. Using RNA-Seq technology, we identified 8,499 differentially expressed genes as temperature responsive in rice cultivar IRBB61 experiencing susceptible and resistant interactions across three time points. Notably, genes in the plant hormone abscisic acid biosynthesis and response pathways were up-regulated by high temperature in both mock-treated plants and plants experiencing a susceptible interaction and were suppressed by high temperature in plants exhibiting Xa7-mediated resistance. Genes responsive to salicylic acid, an important plant hormone for disease resistance, were down-regulated by high temperature during both the susceptible and resistant interactions, suggesting that enhanced Xa7-mediated resistance at high temperature is not dependent on salicylic acid signaling. A DNA sequence motif similar to known abscisic acid-responsive cis-regulatory elements was identified in the promoter region upstream of genes up-regulated in susceptible but down-regulated in resistant interactions. The results of our study suggest that the plant hormone abscisic acid is an important node for cross-talk between plant transcriptional response pathways to high temperature stress and pathogen attack. Genes in this pathway represent an important focus for future study to determine how plants evolved to deal with simultaneous abiotic and biotic stresses.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
5.6
作者：
Lim CW;Baek W;Jung J;Kim JH;Lee SC
通讯作者：
Lee SC
邮票：用于探索DNA结合基序相似性的网络工具。
影响因子：
14.9
作者：
Mahony, Shaun;Benos, Panayiotis V
通讯作者：
Benos, Panayiotis V
拟南芥脱落酸缺乏通过增强抗性蛋白SNC1和RPS4的核积累来拮抗高温抑制抗病性
影响因子：
11.6
作者：
Mang, Hyung-Gon;Qian, Weiqiang;Hua, Jian
通讯作者：
Hua, Jian
影响因子：
1.8
作者：
Madgwick, James W.;West, Jon S.;Fitt, Bruce D. L.
通讯作者：
Fitt, Bruce D. L.