Identification of sulfated oligosialic acid units in the O-linked glycan of the sea urchin egg receptor for sperm.
Identification of sulfated oligosialic acid units in the O-linked glycan of the sea urchin egg receptor for sperm.
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海胆卵子精子受体 O 连接聚糖中硫酸化寡唾液酸单元的鉴定。
DOI：
10.1073/pnas.94.8.3650
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发表时间：
1997
影响因子：
11.1
通讯作者：
Lennarz,WJ
中科院分区：
文献类型：
--
作者：
Kitazume-Kawaguchi,S;Inoue,S;Inoue,Y;Lennarz,WJ
紫球海胆精子卵受体是一种分子质量为350 kDa的细胞表面糖蛋白。最近的研究表明，从受体中分离出来的硫化O-连接的多糖与顶体反应的精子结合。对纯化的受体进行了氨基酸和碳水化合物含量的分析，表明其由70%的碳水化合物重量组成。组成分析表明，N-ANDO连接的低聚糖链均存在。在多肽：N-葡聚糖酶处理受体后，去除了大部分当时连接的糖链，大多数唾液酸残基仍然与受体相关，几种类型的实验表明，这些唾液酸基是由连接到受体的O-连接的糖链上的低聚唾液酸单元组成的。早些时候从另一种海胆的卵细胞表面复合体中分离得到的糖肽组分中发现的寡唾液链的化学和物理研究证实，这些分子具有如下结构：(SO_4−)-9Neu5Gcα2(→5-OlycolylNeu5Gcα2→)n。通过比较和分析研究，得出结论：这种硫化的寡糖是含有GalNAc的链的组成部分，该链与精子的海胆卵受体的多肽链ISO连接。用竞争性受精抑制法检测表明，硫代低聚唾液酸链来源于THs。紫癜性卵细胞表面复合体抑制受精；这种寡唾液酸链的非硫酸形式几乎没有抑制活性。
TheStrongylocentrotus purpuratussea urchin egg receptor for sperm is a cell surface glycoprotein with a molecular mass of 350 kDa. Recent studies indicate that the sulfatedO-linked glycans isolated from the receptor bind to acrosome-reacted sperm. The purified receptor was analyzed with respect to amino acid and carbohydrate content and shown to be composed of 70% carbohydrate by weight. Compositional analysis indicated that bothN- andO-linked oligosaccharide chains were present. After peptide:N-glycanase treatment of the receptor to remove most of theN-linked glycan chains, the majority of the sialic acid residues remained associated with the receptor and were shown by several types of experiments to be composed of sulfated oligosialic acid units attached to theO-linked glycan chains of the receptor. Chemical and physical studies on oligosialic chains discovered earlier in the Pronase-generated glycopeptide fraction isolated from the egg cell surface complex of another species of sea urchin,Hemicentrotus pulcherrimus, established that these molecules had the structure: (SO4−)-9Neu5Gcα2(→5-OglycolylNeu5Gcα2→)n. Based on comparative and analytical studies, it was concluded that this sulfated oligosaccharide is a component of a GalNAc-containing chain that isO-linked to the polypeptide chain of the sea urchin egg receptor for sperm. Using a competitive inhibition of fertilization bioassay it was shown that the sulfated oligosialic acid chains derived from theS. purpuratusegg cell surface complex inhibited fertilization; the nonsulfated form of this oligosialic chain had little inhibitory activity.
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海胆配子的激活。
DOI：
--
发表时间：
1986
期刊：
Annual Review of Cell Biology
影响因子：
--
作者：
J. Trimmer;V. Vacquier
通讯作者：
V. Vacquier
脑膜炎奈瑟菌 B 组和 C 组多糖的决定性特异性。
影响因子：
4.4
作者：
H. Jennings;R. Roy;F. Michon
通讯作者：
F. Michon
220 kDa 卵黄膜糖蛋白通过 O 连接寡糖介导 Unio elongatulus 极化卵子中精子的结合。
影响因子：
2.7
作者：
R. Focarelli;F. Rosati
通讯作者：
F. Rosati
从海胆 Hemicentrotus pulcherrimus 卵中纯化精子结合因子。
DOI：
10.1016/0006-291x(77)90753-7
发表时间：
1977
影响因子：
3.1
作者：
H. Tsuzuki;M. Yoshida;K. Onitake;K. Aketa
通讯作者：
K. Aketa
DOI：
10.1016/0304-4165(93)90018-4
发表时间：
1993-11-28
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
NOGUCHI, S;NAKANO, M
通讯作者：
NAKANO, M