The role of ORC4 in enucleation of Murine Erythroleukemia (MEL) cells is similar to that in oocyte polar body extrusion.
The role of ORC4 in enucleation of Murine Erythroleukemia (MEL) cells is similar to that in oocyte polar body extrusion.
复制标题
DOI：
10.1080/19396368.2020.1822458
复制
发表时间：
2020-12
影响因子：
2.4
通讯作者：
Ward WS
中科院分区：
文献类型：
--
作者：
Nguyen H;Ung A;Ward WS
起源复制复合物亚基4（ORC 4）是起源复制复合物（ORCs）的六个亚基之一，其对于在DNA复制起点处启动许可和募集各种细胞过程所必需的衔接分子是必不可少的。以前，我们报道过ORC 4在卵子发生过程中的极体排出（PBE）中也起着至关重要的作用，其中一半的染色体从卵母细胞中排出。我们假设ORC 4可能在染色质消除中发挥更广泛的作用。我们测试了它在红细胞发育过程中的去核作用。鼠红白血病（MEL）细胞可以在培养物中无限增殖，并且可以通过用马林-1处理诱导其DNA去核，从而模拟正常的红细胞去核。我们发现，ORC 4出现在MEL细胞的细胞核周围与tetraolin-1处理，去核前厚度逐渐增加。然后，我们通过用siRNA-0 RC 4在拟南芥素-1处理期间下调0 RC 4来测试0 RC 4是否是MEL去核所需的，并且发现这阻止了MEL去核。这些数据与ORC 4是成红细胞去核所需的模型一致，就像卵母细胞PBE一样。他们提出了一种新的模型，其中ORC 4表达是启动去核途径的标志物。
The Origin Replication Complex subunit 4 (ORC4) is one in six subunits of the Origin Replication Complexes (ORCs) which is essential for initiating licensing at DNA replication origins and recruiting adaptor molecules necessary for various cellular processes. Previously, we reported that ORC4 also plays a vital role in polar body extrusion (PBE) during oogenesis in which half the chromosomes are extruded from the oocyte. We hypothesized that ORC4 might play a broader role in chromatin elimination. We tested its role in enucleation during the development of erythrocytes. Murine erythroleukemia (MEL) cells can be propagated in culture indefinitely and can be induced to enucleate their DNA by treatment with Vacuolin-1, thereby mimicking normal erythrocyte enucleation. We found that ORC4 appeared around the nuclei of the MEL cells with Vacuolin-1 treatment, gradually increasing in thickness before enucleation. We then tested whether ORC4 was required for MEL enucleation by down regulating ORC4 with siRNA-ORC4 during Vacuolin-1 treatment and found that this prevented MEL enucleation. These data are consistent with the model that ORC4 is required for erythroblast enucleation just as it is for oocyte PBE. They suggest a new model in which ORC4 expression is a marker for the initiation to the enucleation pathway.
登录
查看更多内容
鼠红血症细胞的末端分化：终阶段细胞的物理稳定。
影响因子：
7.8
作者：
Volloch, V;Housman, D
通讯作者：
Housman, D
DOI：
10.1385/0-89603-178-0:421
发表时间：
1991-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Antoniou, M
通讯作者：
Antoniou, M
DOI：
10.1083/jcb.105.6.3105
发表时间：
1987-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Patel VP;Lodish HF
通讯作者：
Lodish HF
影响因子：
20.3
作者：
Keerthivasan, Ganesan;Small, Sara;Crispino, John D.
通讯作者：
Crispino, John D.
影响因子：
64.5
作者：
Gromley, A;Yeaman, C;Doxsey, SJ
通讯作者：
Doxsey, SJ