High-throughput neuraminidase substrate specificity study of human and avian influenza A viruses.
High-throughput neuraminidase substrate specificity study of human and avian influenza A viruses.
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DOI：
10.1016/j.virol.2011.03.024
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发表时间：
2011-06-20
期刊：
影响因子：
3.7
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Li Y;Cao H;Dao N;Luo Z;Yu H;Chen Y;Xing Z;Baumgarth N;Cardona C;Chen X
尽管神经氨酸酶(NA)活性在甲型流感病毒的有效感染中具有重要作用，但关于不同宿主或不同毒株的病毒神经氨酸酶的底物偏好差异的信息有限。采用高通量筛选方法和20个α2-3或α2-6标记的唾液酸半乳糖苷的文库，用完整的病毒颗粒研究了NAS对37株人和禽流感病毒的底物特异性。所有被测病毒的神经氨酸酶都能切割α2-3和α2-6连接的唾液酸苷，但更倾向于α2-3连接的唾液酸苷，其活性依赖于末端唾液酸结构。与其他亚型甲型流感病毒不裂解2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic酸(KdN)或5-脱氧kdN(5d-kdN)的Nas不同，所有N7亚型病毒的Nas对含有kdN或5d-kdN的α2-3连接唾液酸具有显著的水解性。此外，第1组Nas还显示了从α2-3连接的唾液酸中裂解N-叠氮乙酰神经氨酸的活性。
Despite the importance of neuraminidase (NA) activity in effective infection by influenza A viruses, limited information exists about the differences of substrate preferences of viral neuraminidases from different hosts or from different strains. Using a high-throughput screening format and a library of twenty α2–3- or α2–6-linked para-nitrophenol-tagged sialylgalactosides, substrate specificity of NAs on thirty-seven strains of human and avian influenza A viruses was studied using intact viral particles. Neuraminidases of all viruses tested cleaved both α2–3- and α2–6-linked sialosides but preferred α2–3-linked ones and the activity was dependent on the terminal sialic acid structure. In contrast to NAs of other subtypes of influenza A viruses which did not cleave 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid (Kdn) or 5-deoxy Kdn (5d–Kdn), NAs of all N7 subtype viruses tested had noticeable hydrolytic activities on α2–3-linked sialosides containing Kdn or 5d–Kdn. Additionally, group 1 NAs showed efficient activity in cleaving N-azidoacetylneuraminic acid from α2 –3-linked sialoside.
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影响因子：
4
作者：
Chen, Xi;Varki, Ajit
通讯作者：
Varki, Ajit
影响因子：
3.7
作者：
PALESE, P;TOBITA, K;COMPANS, RW
通讯作者：
COMPANS, RW
影响因子：
5.4
作者：
Mitnaul, LJ;Matrosovich, MN;Kawaoka, Y
通讯作者：
Kawaoka, Y
影响因子：
9.4
作者：
Butt, KM;Smith, GJD;Guan, Y
通讯作者：
Guan, Y
DOI：
10.1073/pnas.160270697
发表时间：
2000-08-15
影响因子：
11.1
作者：
Lin, YP;Shaw, M;Hay, A
通讯作者：
Hay, A