Harnessing physicochemical properties of virus capsids for designing enzyme confined nanocompartments.
Harnessing physicochemical properties of virus capsids for designing enzyme confined nanocompartments.
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DOI：
10.1016/j.coviro.2021.12.012
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发表时间：
2022-03
影响因子：
5.9
通讯作者：
Lovell L
中科院分区：
文献类型：
--
作者：
Uchida M;Manzo E;Echeveria D;Jiménez S;Lovell L
病毒由于其优雅的结构和微妙的平衡活动，引起了病毒学以外的各种学科的重大科学兴趣。病毒样颗粒（VLP）是一种来自病毒或其他笼形成蛋白的非感染性蛋白笼，已被用作生物启发工程和合成操作的纳米级平台，具有一系列应用。功能性蛋白质，特别是酶的包封是VLP的一种新兴用途，其不仅有希望用于开发高效和稳健的催化材料，而且还提供了对细胞中通常观察到的酶区室化效应的基本见解。这篇综述强调了最近的进展，采用VLPs作为容器限制酶。为了实现更大和更可控的酶加载，已经开发了各种不同的酶封装策略;这些策略中的许多策略受到病毒衣壳的组装和基因组加载机制的启发。表征VLP的物理化学性质，如孔隙率，可以导致合理的操作和更好地理解材料的催化行为。
Viruses have drawn significant scientific interest from a wide variety of disciplines beyond virology because of their elegant architectures and delicately balanced activities. A virus-like particle (VLP), a noninfectious protein cage derived from viruses or other cage-forming proteins, has been exploited as a nano-scale platform for bioinspired engineering and synthetic manipulation with a range of applications. Encapsulation of functional proteins, especially enzymes, is an emerging use of VLPs that is promising not only for developing efficient and robust catalytic materials, but also for providing fundamental insights into the effects of enzyme compartmentalization commonly observed in cells. This review highlights recent advances in employing VLPs as a container for confining enzymes. To accomplish larger and more controlled enzyme loading, various different enzyme encapsulation strategies have been developed; many of these strategies are inspired from assembly and genome loading mechanisms of viral capsids. Characterization of VLPs’ physicochemical properties, such as porosity, could lead to rational manipulation and a better understanding of the catalytic behavior of the materials.
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