Alterations in diversity of the oral microbiome in pediatric inflammatory bowel disease.
Alterations in diversity of the oral microbiome in pediatric inflammatory bowel disease.
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DOI：
10.1002/ibd.21874
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发表时间：
2012-05
影响因子：
4.9
通讯作者：
Bousvaros, Athos
中科院分区：
文献类型：
--
作者：
Docktor, Michael J.;Paster, Bruce J.;Abramowicz, Shelly;Ingram, Jay;Wang, Yaoyu E.;Correll, Mick;Jiang, Hongyu;Cotton, Sean L.;Kokaras, Alexis S.;Bousvaros, Athos
口腔病理学是克罗恩病（CD）常见的肠外表现。宿主-微生物相互作用与遗传易感宿主的炎症性肠病（IBD）的发病机制有关，但有关IBD中口腔微生物的信息有限。我们假设IBD患者的口腔微生物学可能不同。我们的实验室开发了一种基于16 S rRNA的技术，称为人类口腔微生物识别微阵列（HOMIM），用于研究IBD儿童和年轻人的口腔微生物组。使用HOMIM分析健康对照、CD和溃疡性结肠炎（UC）患者的舌和颊粘膜刷拭。采用Shannon多样性指数（SDI）和主成分分析（PCA）比较了各研究组间种群和门级的变化。总共招募了来自波士顿儿童医院的114名独特受试者。与健康对照的相同位置相比，CD患者的舌样品显示总体微生物多样性显著降低（P = 0.015），梭菌门（P < 0.0002）和厚壁菌门（P = 0.022）中观察到显著变化。与健康对照组相比，UC患者的舌样本在总体微生物多样性方面没有显示出显著变化（P = 0.418）。通过HOMIM检测，我们发现儿童CD的口腔微生物组的总体多样性显著降低。考虑到IBD中提出的微生物-宿主相互作用，口腔的可视化和直接口腔粘膜取样的容易性，IBD中口腔微生物组的进一步研究具有潜在的诊断和预后价值。
Oral pathology is a commonly reported extraintestinal manifestation of Crohn’s disease (CD). The host–microbe interaction has been implicated in the pathogenesis of inflammatory bowel disease (IBD) in genetically susceptible hosts, yet limited information exists about oral microbes in IBD. We hypothesize that the microbiology of the oral cavity may differ in patients with IBD. Our laboratory has developed a 16S rRNA-based technique known as the Human Oral Microbe Identification Microarray (HOMIM) to study the oral microbiome of children and young adults with IBD. Tongue and buccal mucosal brushings from healthy controls, CD, and ulcerative colitis (UC) patients were analyzed using HOMIM. Shannon Diversity Index (SDI) and Principal Component Analysis (PCA) were employed to compare population and phylum-level changes among our study groups. In all, 114 unique subjects from the Children’s Hospital Boston were enrolled. Tongue samples from patients with CD showed a significant decrease in overall microbial diversity as compared with the same location in healthy controls (P = 0.015) with significant changes seen in Fusobacteria (P < 0.0002) and Firmicutes (P = 0.022). Tongue samples from patients with UC did not show a significant change in overall microbial diversity as compared with healthy controls (P = 0.418). As detected by HOMIM, we found a significant decrease in overall diversity in the oral microbiome of pediatric CD. Considering the proposed microbe–host interaction in IBD, the ease of visualization and direct oral mucosal sampling of the oral cavity, further study of the oral microbiome in IBD is of potential diagnostic and prognostic value.
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影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
9.8
作者：
Dubinsky, MC;Lin, YC;Yang, HY
通讯作者：
Yang, HY
影响因子：
0.6
作者：
Papp, Maria;Altorjay, Istvan;Lakatos, Peter Laszlo
通讯作者：
Lakatos, Peter Laszlo
影响因子：
4.9
作者：
Martinez-Medina, Margarita;Aldeguer, Xavier;Garcia-Gil, L. Jesus
通讯作者：
Garcia-Gil, L. Jesus
影响因子：
--
作者：
Dupuy, A;Cosnes, J;Cosnes, A
通讯作者：
Cosnes, A