Reconstruction of distinct vertebrate gastrulation modes via modulation of key cell behaviors in the chick embryo.
Reconstruction of distinct vertebrate gastrulation modes via modulation of key cell behaviors in the chick embryo.
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DOI：
10.1126/sciadv.abn5429
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发表时间：
2023-01-04
期刊：
影响因子：
13.6
通讯作者：
中科院分区：
文献类型：
--
作者：
驱动中胚层和内胚层内化的原肠胚形成的形态在脊椎动物物种中显著不同。它的范围从两栖动物的上皮细胞片通过圆形囊胚孔的退化到间充质细胞通过原始条纹的侵入。通过靶向控制鸡胚中关键细胞行为的信号通路，我们产生了细胞可以或不能进入的新月形和环形中内胚层区域。这些改变颠覆了鸡原条的形成，使之成为两栖动物、爬行动物和硬骨鱼中的原肠胚形成模式。我们的实验操作是由一个理论框架连接细胞行为的自组织多细胞流在所附的文件中详细概述。总而言之，这表明原肠胚形成运动的进化很大程度上取决于不同物种中内胚层区域中一些关键细胞行为的变化，并由相对较少的信号通路控制。中内胚层领域和细胞行为的操作重建不同的脊椎动物原肠胚形态在小鸡。
The morphology of gastrulation driving the internalization of the mesoderm and endoderm differs markedly among vertebrate species. It ranges from involution of epithelial sheets of cells through a circular blastopore in amphibians to ingression of mesenchymal cells through a primitive streak in amniotes. By targeting signaling pathways controlling critical cell behaviors in the chick embryo, we generated crescent- and ring-shaped mesendoderm territories in which cells can or cannot ingress. These alterations subvert the formation of the chick primitive streak into the gastrulation modes seen in amphibians, reptiles, and teleost fish. Our experimental manipulations are supported by a theoretical framework linking cellular behaviors to self-organized multicellular flows outlined in detail in the accompanying paper. Together, this suggests that the evolution of gastrulation movements is largely determined by changes in a few critical cell behaviors in the mesendoderm territory across different species and controlled by a relatively small number of signaling pathways. Manipulation of mesendoderm territory and cell behavior recreates distinct vertebrate gastrulation morphologies in the chick.
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DOI：
10.1242/dev.081901
发表时间：
2012-11
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Bertocchini F;Stern CD
通讯作者：
Stern CD
影响因子：
2.4
作者：
Durande, M.;Tlili, S.;Delanoe-Ayari, H.
通讯作者：
Delanoe-Ayari, H.
影响因子：
64.8
作者：
Kyprianou, Christos;Christodoulou, Neophytos;Zernicka-Goetz, Magdalena
通讯作者：
Zernicka-Goetz, Magdalena
影响因子：
11.8
作者：
Acloque, Herve;Ocana, Oscar H.;Matheu, Ander;Rizzoti, Karine;Wise, Clare;Lovell-Badge, Robin;Angela Nieto, M.
通讯作者：
Angela Nieto, M.
影响因子：
--
作者：
Chapman SC;Matsumoto K;Cai Q;Schoenwolf GC
通讯作者：
Schoenwolf GC