Isolation of novel murine maternal mRNAs regulated by cytoplasmic polyadenylation.
Isolation of novel murine maternal mRNAs regulated by cytoplasmic polyadenylation.
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分离受细胞质多聚腺苷酸化调节的新型小鼠母体 mRNA。
DOI：
10.1101/gad.6.7.1202
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发表时间：
1992
影响因子：
10.5
通讯作者：
Strickland,S
中科院分区：
文献类型：
--
作者：
Sallés,FJ;Darrow,AL;O'Connell,ML;Strickland,S
细胞质多聚腺苷酸化元件(CPE)是许多储存的母体mRNA3‘-非翻译区的富含AU的序列。在非洲爪哇，CPE指导这些休眠的mRNAs的减数分裂成熟特异性胞浆多聚腺苷酸化和翻译激活。这项工作表明，CPE在小鼠卵母细胞中控制着类似的调节，并利用这些信息通过聚合酶链式反应(PCR)分离新的母体mRNAs。用简并的CPE引物与小鼠原代卵母细胞的cDNA进行锚定聚合酶链式反应。鉴定出含有典型的多聚腺苷信号AATAAA的克隆。然后使用一种新的PCR测试来确定减数分裂成熟之前和之后各自的mRNAs的聚腺苷酸化状态。两个mRNA，OM-1和OM-2，在成熟时在细胞质上被多腺化。另一种mRNA在成熟过程中没有多聚腺化，尽管它包含多个CPE样元件，表明该序列元件在这段时间不足以进行腺化。直接针对OM-1的反义寡核苷酸显微注射到初级卵母细胞中可以破坏该mRNA，但似乎不会干扰体外成熟。这些实验识别了两个新的母体mRNAs，并建立了一种简单的策略来分离控制减数分裂成熟、受精和小鼠早期发育的其他母体信息。
The cytoplasmic polyadenylation element (CPE) is an AU-rich sequence in the 3'-untranslated region of many stored maternal mRNAs. The CPE directs the meiotic maturation-specific cytoplasmic polyadenylation and translational activation of these dormant mRNAs in Xenopus. The work presented here demonstrates that the CPE controls a similar regulation in mouse oocytes and utilizes the information to isolate novel maternal mRNAs by polymerase chain reaction (PCR). A degenerate CPE primer was used in an anchored PCR reaction with cDNAs from primary mouse oocytes. Clones were identified that contained the canonical polyadenylation signal AATAAA. A novel PCR test was then used to determine the polyadenylation state of the respective mRNAs before and after meiotic maturation. Two mRNAs, OM-1 and OM-2, are cytoplasmically polyadenylated upon maturation. Another mRNA is not polyadenylated during maturation, although it contains multiple CPE-like elements, indicating that this sequence element is not sufficient for adenylation during this time. Microinjection into primary oocytes of antisense oligodeoxynucleotides directed against OM-1 destroys the mRNA but does not appear to interfere with maturation in vitro. These experiments identify two novel maternal mRNAs and establish a simple strategy for isolating other maternal messages that control meiotic maturation, fertilization, and early mouse development.
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哺乳动物卵母细胞体外减数分裂成熟过程中蛋白质合成模式的具体变化。
DOI：
10.1073/pnas.74.2.538
发表时间：
1977
影响因子：
11.1
作者：
R. Schultz;P. M. Wassarman
通讯作者：
P. M. Wassarman
爪蟾卵母细胞寡脱氧核苷酸注射的非特异性效应：对先前 D7 mRNA 消融实验的重新评估。
DOI：
10.1242/dev.110.3.769
发表时间：
1990
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Smith,RC;Bement,WM;Dersch,MA;Dworkin-Rastl,E;Dworkin,MB;Capco,DG
通讯作者：
Capco,DG
DOI：
10.1073/pnas.85.23.8998
发表时间：
1988-12-01
影响因子：
11.1
作者：
FROHMAN, MA;DUSH, MK;MARTIN, GR
通讯作者：
MARTIN, GR
海洋无脊椎动物卵母细胞、卵子和受精卵中的选择性信使 RNA 翻译
影响因子：
2.7
作者：
E. Rosenthal;F. Wilt
通讯作者：
F. Wilt
开始即是结束：早期发育过程中 Poly(A) 添加和去除的调节。
影响因子：
13.8
作者：
Wickens,M
通讯作者：
Wickens,M