Secretome Analysis of the Pine Wood Nematode Bursaphelenchus xylophilus Reveals the Tangled Roots of Parasitism and Its Potential for Molecular Mimicry.
Secretome Analysis of the Pine Wood Nematode Bursaphelenchus xylophilus Reveals the Tangled Roots of Parasitism and Its Potential for Molecular Mimicry.
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DOI：
10.1371/journal.pone.0067377
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Futai K
中科院分区：
文献类型：
--
作者：
Shinya R;Morisaka H;Kikuchi T;Takeuchi Y;Ueda M;Futai K
自世纪初从北美传入亚洲以来，松材线虫引起了毁灭性的森林疾病，称为松树枯萎病。这种新出现的病原体传播到欧洲部分地区，并在葡萄牙和西班牙被发现为松树枯萎病的致病因子。2011年，B的全基因组序列。确定了木霉菌，这使我们能够对B进行更详细的分析。木虱寄生在这里，我们鉴定了B分泌的1,515种蛋白质。利用高灵敏度的蛋白质组学方法结合现有的基因组序列，分泌蛋白的目录包含参与营养吸收、迁移和逃避宿主防御的蛋白。寄生线虫分泌组的比较功能分析揭示了分泌的肽酶和肽酶抑制剂在B中的显著扩增。通过基因复制和来自真菌和细菌的水平基因转移，此外，我们还证明了B.木麻黄分泌的拟寄主蛋白与寄主松树的拟寄主蛋白非常相似。这些蛋白质可能是通过宿主-寄生虫共同进化获得的，并且可能在感染期间模仿易感松树中的宿主防御系统。本研究有助于理解线虫独特的寄生性及其缠绕的根，并为植物线虫寄生性的进化提供了新的视角。
Since it was first introduced into Asia from North America in the early 20th century, the pine wood nematode Bursaphelenchus xylophilus has caused the devastating forest disease called pine wilt. The emerging pathogen spread to parts of Europe and has since been found as the causal agent of pine wilt disease in Portugal and Spain. In 2011, the entire genome sequence of B. xylophilus was determined, and it allowed us to perform a more detailed analysis of B. xylophilus parasitism. Here, we identified 1,515 proteins secreted by B. xylophilus using a highly sensitive proteomics method combined with the available genomic sequence. The catalogue of secreted proteins contained proteins involved in nutrient uptake, migration, and evasion from host defenses. A comparative functional analysis of the secretome profiles among parasitic nematodes revealed a marked expansion of secreted peptidases and peptidase inhibitors in B. xylophilus via gene duplication and horizontal gene transfer from fungi and bacteria. Furthermore, we showed that B. xylophilus secreted the potential host mimicry proteins that closely resemble the host pine’s proteins. These proteins could have been acquired by host–parasite co-evolution and might mimic the host defense systems in susceptible pine trees during infection. This study contributes to an understanding of their unique parasitism and its tangled roots, and provides new perspectives on the evolution of plant parasitism among nematodes.
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影响因子：
7.4
作者：
Guo, Yongfeng;Ni, Jun;Clark, Steven E.
通讯作者：
Clark, Steven E.
影响因子：
4.1
作者：
Fierens, Ellen;Rombouts, Sigrid;Delcour, Jan A.
通讯作者：
Delcour, Jan A.
影响因子：
2.1
作者：
HAWDON, JM;JONES, BF;HOTEZ, PJ
通讯作者：
HOTEZ, PJ
影响因子：
3.8
作者：
Bennuru S;Semnani R;Meng Z;Ribeiro JM;Veenstra TD;Nutman TB
通讯作者：
Nutman TB
影响因子：
1.3
作者：
BIRD, AF
通讯作者：
BIRD, AF