Histone variant H2A.Z promotes meiotic chromosome axis organization in Saccharomyces cerevisiae.
Histone variant H2A.Z promotes meiotic chromosome axis organization in Saccharomyces cerevisiae.
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DOI：
10.1093/g3journal/jkac128
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发表时间：
2022-07-29
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影响因子：
--
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减数分裂的进展与染色体结构的重大重组有关，部分由组蛋白和染色质的变化调节。先前的研究发现，缺乏连接蛋白H1或变异组蛋白H2A.Z的酵母菌株在减数分裂过程中存在缺陷。为了进一步确定这些染色质因子的作用，我们对在没有H1和H2A.Z的情况下进行减数分裂的细胞进行了遗传学和细胞学分析。我们发现在缺乏H2A的菌株中观察到孢子活力缺陷。如果细胞也缺乏组蛋白H1，则Z可以部分抑制，而H1和H2A同时缺失。Z与升高的基因转换事件有关。细胞学分析的Red1和Rec8染色模式表明，缺乏H2A的细胞亚群。Z不能组装正确的染色体轴，并且htz1Δ/htz1Δ细胞中突触复合体蛋白Zip1的染色模式与缺乏rec8依赖性减数分裂内聚的细胞的染色模式相似。我们的结果表明H2A的作用。Z在减数分裂染色体轴的建立或维持中，可能是通过促进染色体的有效内聚。
Progression through meiosis is associated with significant reorganization of chromosome structure, regulated in part by changes in histones and chromatin. Prior studies observed defects in meiotic progression in yeast strains lacking the linker histone H1 or variant histone H2A.Z. To further define the contributions of these chromatin factors, we have conducted genetic and cytological analysis of cells undergoing meiosis in the absence of H1 and H2A.Z. We find that a spore viability defect observed in strains lacking H2A.Z can be partially suppressed if cells also lack histone H1, while the combined loss of both H1 and H2A.Z is associated with elevated gene conversion events. Cytological analysis of Red1 and Rec8 staining patterns indicates that a subset of cells lacking H2A.Z fail to assemble a proper chromosome axis, and the staining pattern of the synaptonemal complex protein Zip1 in htz1Δ/htz1Δ cells mimics that of cells deficient for Rec8-dependent meiotic cohesion. Our results suggest a role for H2A.Z in the establishment or maintenance of the meiotic chromosome axis, possibly by promoting the efficient chromosome cohesion.
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影响因子：
5.6
作者：
Jaiswal D;Turniansky R;Green EM
通讯作者：
Green EM
DOI：
10.1534/g3.115.024380
发表时间：
2016-01-08
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Chu DB;Burgess SM
通讯作者：
Burgess SM
ECM11-GMC2复合物通过在发芽酵母中的横向丝组装来促进突发型复合物的形成。
影响因子：
4.5
作者：
Humphryes N;Leung WK;Argunhan B;Terentyev Y;Dvorackova M;Tsubouchi H
通讯作者：
Tsubouchi H
影响因子：
14.9
作者：
Lee MS;Higashide MT;Choi H;Li K;Hong S;Lee K;Shinohara A;Shinohara M;Kim KP
通讯作者：
Kim KP
影响因子：
5.3
作者：
Bryant, Jessica M.;Govin, Jerome;Berger, Shelley L.
通讯作者：
Berger, Shelley L.