Host-specific signatures of the cell wall changes induced by the plant parasitic nematode, Meloidogyne incognita.
Host-specific signatures of the cell wall changes induced by the plant parasitic nematode, Meloidogyne incognita.
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DOI：
10.1038/s41598-018-35529-7
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发表时间：
2018-11-23
影响因子：
4.6
通讯作者：
Urwin PE
中科院分区：
文献类型：
--
作者：
Bozbuga R;Lilley CJ;Knox JP;Urwin PE
根结线虫（Meloidogyne spp.）是一类重要的植物寄生线虫，其在宿主植物根部内诱导独特的取食位点结构，称为巨细胞，其为线虫提供营养流。对南方根结线虫侵染的拟南芥、玉米和小豆巨细胞壁多糖进行了原位比较分析。所有三个物种的巨细胞壁的共同特征包括存在高酯化果胶同型半乳糖醛酸、木葡聚糖和果胶阿拉伯聚糖。玉米巨细胞壁中木聚糖和混合连接葡聚糖（MLG）表位的种特异性存在反映了宿主的分类学群体。LM5半乳聚糖和LM21甘露聚糖表位在小豆巨细胞壁中未检测到，但在拟南芥和玉米巨细胞壁中检测到。LM2阿拉伯半乳聚糖蛋白表位是值得注意的，其明显的全球变化的根细胞壁作为一个响应感染的三个主机物种。此外，一组拟南芥细胞壁突变体被用来确定改变细胞壁结构对M的任何影响。不明感染阿拉伯半乳聚糖蛋白8基因的破坏具有最大的影响，并导致感染率增加。
Root-knot nematodes (Meloidogyne spp.) are an important group of plant parasitic nematodes that induce within host plant roots unique feeding site structures, termed giant cells, which supply nutrient flow to the nematode. A comparative in situ analysis of cell wall polysaccharides in the giant cells of three host species (Arabidopsis, maize and aduki bean) infected with Meloidogyne incognita has been carried out. Features common to giant cell walls of all three species include the presence of high-esterified pectic homogalacturonan, xyloglucan and pectic arabinan. The species-specific presence of xylan and mixed-linkage glucan (MLG) epitopes in giant cell walls of maize reflected that host’s taxonomic group. The LM5 galactan and LM21 mannan epitopes were not detected in the giant cell walls of aduki bean but were detected in Arabidopsis and maize giant cell walls. The LM2 arabinogalactan-protein epitope was notable for its apparent global variations in root cell walls as a response to infection across the three host species. Additionally, a set of Arabidopsis cell wall mutants were used to determine any impacts of altered cell wall structures on M. incognita infection. Disruption of the arabinogalactan-protein 8 gene had the greatest impact and resulted in an increased infection rate.
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