Characterization of the glycoproteins of bat-derived influenza viruses.
Characterization of the glycoproteins of bat-derived influenza viruses.
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DOI：
10.1016/j.virol.2015.11.002
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发表时间：
2016-01-15
期刊：
影响因子：
3.7
通讯作者：
Takada A
中科院分区：
文献类型：
--
作者：
Maruyama J;Nao N;Miyamoto H;Maeda K;Ogawa H;Yoshida R;Igarashi M;Takada A
最近发现的蝙蝠衍生的流感病毒（BatIV）具有与先前已知的甲型流感病毒不同的血凝素（HA）和神经氨酸酶（NA）基因片段。然而，这些BatIV的致病性仍然未知，因为尚未分离出感染性病毒株。为了深入了解BatIV的生物学特性，我们产生了用BatIV HA和NA假型化的水泡性口炎病毒（VSV）。我们发现，VSV假型与蝙蝠IV HA和NA有效地感染特定的蝙蝠细胞系，但不是那些来自灵长类动物，和胰蛋白酶样蛋白酶的蛋白水解裂解是必要的HA介导的病毒进入。用一些酶和抑制剂处理敏感的蝙蝠细胞显示，BatIV HA可能识别一些细胞糖蛋白作为受体，而不是用于其他已知流感病毒的唾液酸。这些数据提供了关于BatIV进入细胞和宿主限制的机制的基本信息。特定的蝙蝠细胞系可能对蝙蝠流感病毒敏感。蝙蝠流感病毒不使用唾液酸受体。一些细胞类型特异性分子可以作为蝙蝠流感病毒受体。蝙蝠流感病毒可能具有有限的宿主范围。
Recently found bat-derived influenza viruses (BatIVs) have hemagglutinin (HA) and neuraminidase (NA) gene segments distinct from those of previously known influenza A viruses. However, pathogenicities of these BatIVs remain unknown since infectious virus strains have not been isolated yet. To gain insight into the biological properties of BatIVs, we generated vesicular stomatitis viruses (VSVs) pseudotyped with the BatIV HA and NA. We found that VSVs pseudotyped with BatIV HAs and NAs efficiently infected particular bat cell lines but not those derived from primates, and that proteolytic cleavage with a trypsin-like protease was necessary for HA-mediated virus entry. Treatment of the susceptible bat cells with some enzymes and inhibitors revealed that BatIV HAs might recognize some cellular glycoproteins as receptors rather than the sialic acids used for the other known influenza viruses. These data provide fundamental information on the mechanisms underlying the cellular entry and host restriction of BatIVs. Particular bat cell lines may be susceptible to bat influenza viruses. Bat influenza viruses do not use sialic acid receptors. Some cell type-specific molecules may serve as bat influenza virus receptors. Bat influenza viruses may have a limited host range.
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影响因子：
3.7
作者：
Muramatsu M;Yoshida R;Miyamoto H;Tomabechi D;Kajihara M;Maruyama J;Kimura T;Manzoor R;Ito K;Takada A
通讯作者：
Takada A
DOI：
10.1073/pnas.1211037109
发表时间：
2012-11-13
影响因子：
11.1
作者：
Li, Qing;Sun, Xiaoman;Gao, George F.
通讯作者：
Gao, George F.
影响因子：
3.7
作者：
LAZAROWI.SG;COMPANS, RW;CHOPPIN, PW
通讯作者：
CHOPPIN, PW
影响因子：
8
作者：
COLMAN, PM
通讯作者：
COLMAN, PM
影响因子：
3.7
作者：
Makarova, NV;Ozaki, H;Perez, DR
通讯作者：
Perez, DR