Fabrication and Characterization of Micropatterned Living Cells
Fabrication and Characterization of Micropatterned Living Cells
复制标题
微图案活细胞的制造和表征
DOI：
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
T. Matsue
中科院分区：
文献类型：
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作者：
M. Nishizawa;H. Kaji;K. Takoh;T. Matsue
最近对细胞黏附分子机制的研究进展促使人们研究设计界面来在μm尺度上调节细胞黏附。这种细胞微图案化已经被许多研究小组广泛尝试，因为细胞黏附和生长的空间控制是许多生物技术领域的关键问题，如组织工程、基于细胞的生物分析和生物电子学。在此，我们报告了我们在1)细胞微图案化技术的发展和2)微图案化细胞和细胞网络功能的表征方面的最新结果。应用著名的“微接触印刷法”和最新发展的“电化学光刻”技术来制备单细胞比对。细胞的活动受细胞形状的调节。这些有图案的心肌细胞通过缝隙连接相互连接，并作为心脏组织的药理学模型。
Recent progress in understanding molecular mechanism of cell adhesion has prompted researches for designing interfaces to regulate cell adhesion in μm scale. Such cellular micropatterning has been extensively attempted by a number of research groups, since the spatial control of cellular adhesion and growth is a critical issue in many areas of biotechnology such as tissue engineering, cell-based bioanalysis and bioelectronics. We report herein our recent results on 1) the development of techniques for cellular micropatterning and 2) the characterization of the functions of micropatterned cells and cellular networks. The well-known “microcontact-printing” andthe newly developed “electrochemical lithography” were applied to prepare the single-cell alignments. The cellular activity was found to be regulated by the cell shape. The patterned cardiomyocytes connected each other via gap junctions and served as a pharmacological model of cardiac tissue.
影响因子：
56.9
作者：
Chen, CS;Mrksich, M;Ingber, DE
通讯作者：
Ingber, DE
影响因子：
14
作者：
Kane, RS;Takayama, S;Whitesides, GM
通讯作者：
Whitesides, GM