Diversity of transgenic mouse models for selective targeting of midbrain dopamine neurons.
Diversity of transgenic mouse models for selective targeting of midbrain dopamine neurons.
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DOI：
10.1016/j.neuron.2014.12.036
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发表时间：
2015-01-21
期刊：
影响因子：
16.2
通讯作者：
Malenka, Robert C.
中科院分区：
文献类型：
--
作者：
Lammel, Stephan;Steinberg, Elizabeth E.;Foeldy, Csaba;Wall, Nicholas R.;Beier, Kevin;Luo, Liqun;Malenka, Robert C.
中脑被盖区（VTA）多巴胺（DA）神经元与奖赏、厌恶、突显、认知和几种神经精神疾病有关。涉及转基因Cre-driver小鼠系的光遗传学方法为解剖DA特异性功能提供了有力的工具。然而，VTA电路的新兴复杂性需要Cre驱动小鼠系，其将转基因表达限制在精确定义的细胞群中。由于最近的工作报告，腹侧被盖区DA神经元投射到外侧缰释放GABA，但不是DA，我们进行了广泛的解剖，分子和功能表征突出的DA转基因小鼠驱动线。我们发现，在酪氨酸羟化酶，但不是多巴胺转运蛋白，启动子的控制下，转基因表现出显着的非DA细胞特异性的表达模式内和周围的VTA核。我们的研究结果表明，如何在无意中靶向的细胞在转基因小鼠系Cre表达可以混淆的解释，假定细胞类型特异性实验。这篇论文是对发表在《神经元》上的论文的回应。另请参阅与《神经元中出现的问题》同时发表的《引起反应的问题》论文。
Ventral tegmental area (VTA) dopamine (DA) neurons have been implicated in reward, aversion, salience, cognition, and several neuropsychiatric disorders. Optogenetic approaches involving transgenic Cre-driver mouse lines provide powerful tools for dissecting DA-specific functions. However, the emerging complexity of VTA circuits requires Cre-driver mouse lines that restrict transgene expression to a precisely defined cell population. Because of recent work reporting that VTA DA neurons projecting to the lateral habenula release GABA, but not DA, we performed an extensive anatomical, molecular, and functional characterization of prominent DA transgenic mouse driver lines. We find that transgenes under control of the tyrosine hydroxylase, but not the dopamine transporter, promoter exhibit dramatic non-DA cell-specific expression patterns within and around VTA nuclei. Our results demonstrate how Cre expression in unintentionally targeted cells in transgenic mouse lines can confound the interpretation of supposedly cell-type-specific experiments. This Matters Arising paper is in response to, published in Neuron. See also the Matters Arising Response paper by, published concurrently with this Matters Arising in Neuron.
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DOI：
10.1126/science.1249240
发表时间：
2014-04-18
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Friedman AK;Walsh JJ;Juarez B;Ku SM;Chaudhury D;Wang J;Li X;Dietz DM;Pan N;Vialou VF;Neve RL;Yue Z;Han MH
通讯作者：
Han MH
影响因子：
4.7
作者：
Lammel S;Lim BK;Malenka RC
通讯作者：
Malenka RC
影响因子：
5.3
作者：
Ilango, Anton;Kesner, Andrew J.;Ikemoto, Satoshi
通讯作者：
Ikemoto, Satoshi
DOI：
10.1126/science.1232437
发表时间：
2013-04-12
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Kim TI;McCall JG;Jung YH;Huang X;Siuda ER;Li Y;Song J;Song YM;Pao HA;Kim RH;Lu C;Lee SD;Song IS;Shin G;Al-Hasani R;Kim S;Tan MP;Huang Y;Omenetto FG;Rogers JA;Bruchas MR
通讯作者：
Bruchas MR
影响因子：
3.7
作者：
Margolis EB;Toy B;Himmels P;Morales M;Fields HL
通讯作者：
Fields HL