Influence of milk-feeding type and genetic risk of developing coeliac disease on intestinal microbiota of infants: the PROFICEL study.
Influence of milk-feeding type and genetic risk of developing coeliac disease on intestinal microbiota of infants: the PROFICEL study.
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DOI：
10.1371/journal.pone.0030791
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Sanz Y
中科院分区：
文献类型：
--
作者：
Palma GD;Capilla A;Nova E;Castillejo G;Varea V;Pozo T;Garrote JA;Polanco I;López A;Ribes-Koninckx C;Marcos A;García-Novo MD;Calvo C;Ortigosa L;Peña-Quintana L;Palau F;Sanz Y
环境因素和易感基因之间的相互作用可能参与了乳糜泻（CD）的发展。这项研究评估了母乳喂养类型和HLA基因型是否影响有CD家族史的婴儿的肠道微生物群组成。研究包括164名健康新生儿，至少有一个一级亲属患有CD，根据他们的HLA-DQ基因型进行分类，通过PCR-SSP DQB 1和DQA 1分型。通过定量PCR分析7天、1月龄和4月龄时的粪便微生物群。应用线性混合模型重复测量分析，未发现哺乳类型和HLA-DQ基因型对细菌数量的显著交互作用。在整个种群中，母乳喂养促进了C. leptum组，B. longum和B.短拟杆菌、脆弱拟杆菌、C.球孢E. rectale group，E. coli和B.乳酸。此外，增加了B的数量。fragilis组和葡萄球菌属，和减少的双歧杆菌属（Bifidobacterium spp.）和B。在患有CD的遗传风险增加的婴儿中检测到longum。对母乳喂养或配方奶粉喂养婴儿亚组的分析表明，在这两种情况下，CD风险增加与B数量减少有关。和/或双歧杆菌属（Bifidobacterium spp.）此外，在母乳喂养的婴儿中，发生CD的遗传风险增加与C。而在配方奶粉喂养的婴儿中，它与葡萄球菌和B的增加有关。fragilis组编号。总体而言，母乳喂养类型与HLA-DQ基因型结合在建立婴儿肠道微生物群方面发挥着作用;此外，母乳喂养减少了微生物群组成中与基因型相关的差异，这可以部分解释母乳在这种疾病中的保护作用。
Interactions between environmental factors and predisposing genes could be involved in the development of coeliac disease (CD). This study has assessed whether milk-feeding type and HLA-genotype influence the intestinal microbiota composition of infants with a family history of CD. The study included 164 healthy newborns, with at least one first-degree relative with CD, classified according to their HLA-DQ genotype by PCR-SSP DQB1 and DQA1 typing. Faecal microbiota was analysed by quantitative PCR at 7 days, and at 1 and 4 months of age. Significant interactions between milk-feeding type and HLA-DQ genotype on bacterial numbers were not detected by applying a linear mixed-model analysis for repeated measures. In the whole population, breast-feeding promoted colonization of C. leptum group, B. longum and B. breve, while formula-feeding promoted that of Bacteroides fragilis group, C. coccoides-E. rectale group, E. coli and B. lactis. Moreover, increased numbers of B. fragilis group and Staphylococcus spp., and reduced numbers of Bifidobacterium spp. and B. longum were detected in infants with increased genetic risk of developing CD. Analyses within subgroups of either breast-fed or formula-fed infants indicated that in both cases increased risk of CD was associated with lower numbers of B. longum and/or Bifidobacterium spp. In addition, in breast-fed infants the increased genetic risk of developing CD was associated with increased C. leptum group numbers, while in formula-fed infants it was associated with increased Staphylococcus and B. fragilis group numbers. Overall, milk-feeding type in conjunction with HLA-DQ genotype play a role in establishing infants' gut microbiota; moreover, breast-feeding reduced the genotype-related differences in microbiota composition, which could partly explain the protective role attributed to breast milk in this disorder.
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影响因子：
2.3
作者：
Bezirtzoglou, Eugenia;Tsiotsias, Arsenis;Welling, Gjalt W.
通讯作者：
Welling, Gjalt W.
影响因子：
4.9
作者：
Frank, Daniel N.;Robertson, Charles E.;Hamm, Christina M.;Kpadeh, Zegbeh;Zhang, Tianyi;Chen, Hongyan;Zhu, Wei;Sartor, R. Balfour;Boedeker, Edgar C.;Harpaz, Noam;Pace, Norman R.;Li, Ellen
通讯作者：
Li, Ellen
影响因子：
4.4
作者：
Martin, Rocio;Jimenez, Esther;Rodriguez, Juan M.
通讯作者：
Rodriguez, Juan M.
影响因子：
4.4
作者：
Matsuki, T;Watanabe, K;Tanaka, R
通讯作者：
Tanaka, R
影响因子：
7.1
作者：
Ivarsson, A;Hernell, O;Persson, LÅ
通讯作者：
Persson, LÅ