Immunolocalization of the voltage-gated potassium channel Kv2.2 in GABAergic neurons in the basal forebrain of rats and mice.
Immunolocalization of the voltage-gated potassium channel Kv2.2 in GABAergic neurons in the basal forebrain of rats and mice.
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DOI：
10.1002/cne.22457
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发表时间：
2010-11-01
影响因子：
2.5
通讯作者：
Misonou, Hiroaki
中科院分区：
文献类型：
--
作者：
Hermanstyne, Tracey O.;Kihira, Yoshitaka;Misono, Kaori;Deitchler, Ashley;Yanagawa, Yuchio;Misonou, Hiroaki
Kv2 电压门控钾通道 Kv2.1 和 Kv2.2 是哺乳动物大脑中神经元兴奋性的重要调节因子。研究表明，Kv2.1 通道几乎在大脑的所有神经元中表达。然而，Kv2.2 的细胞定位尚未完全阐明。在本文中，我们报告Kv2.2在基底前脑复合体的大细胞视前核（MCPO）和布罗卡对角带（HDB）的水平肢的神经元子集中高表达，这些区域与皮质活动和睡眠/觉醒周期的调节密切相关。研究表明，MCPO 和 HDB 含有不同的神经元群，这些神经元的神经化学物质、胆碱能、谷氨酸和 GABA 能神经元有所不同。使用在 GABA 能神经元中表达 GFP 的特异性免疫标记和敲入小鼠，我们发现 Kv2.2 在 MCPO 和 HDB 的 GABA 能神经元的大量亚群中大量表达。这些数据提供 Kv2.2 作为研究基底前脑 GABA 能神经元特定亚群作用的分子靶点。
The Kv2 voltage-gated potassium channels, Kv2.1 and Kv2.2, are important regulators of neuronal excitability in mammalian brain. It has been shown that Kv2.1 channels are expressed in virtually all neurons in the brain. However, the cellular localization of Kv2.2 has not been fully elucidated. In this paper, we report that Kv2.2 is highly expressed in a subset of neurons in the magnocellular preoptic nucleus (MCPO) and the horizontal limb of the diagonal band of Broca (HDB) of the basal forebrain complex, which are areas highly implicated in the regulation of cortical activity and the sleep/wake-cycle. It has been shown that the MCPO and HDB contain distinct populations of neurons that differ in their neurochemicals, cholinergic, glutamatergic, and GABAergic neurons. Using specific immunolabeling and knock-in mice in which GFP is expressed in GABAergic neurons, we found that Kv2.2 is abundantly expressed in a large sub-population of the GABAergic neurons in the MCPO and HDB. These data offer Kv2.2 as a molecular target to study the role of the specific sub-population of basal forebrain GABAergic neurons.
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