Translocator Protein (TSPO) as a Potential Biomarker in Human Cancers.
Translocator Protein (TSPO) as a Potential Biomarker in Human Cancers.
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DOI：
10.3390/ijms19082176
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发表时间：
2018-07-25
影响因子：
5.6
通讯作者：
Dlamini Z
中科院分区：
文献类型：
--
作者：
Bhoola NH;Mbita Z;Hull R;Dlamini Z
TSPO是一种参与细胞增殖、凋亡和线粒体功能调节的受体。以往的研究表明，TSPO蛋白的表达与肿瘤的恶性程度呈正相关，与患者的生存期呈负相关。本研究的目的是检测TSPO在不同类型的正常组织和癌组织中的转录水平。用原位杂交的方法定位TSPO在人类正常组织和癌组织中的表达。用荧光强度和比色目测法测定TSPO mRNA的相对水平。用RT-PCR证实这些基因在正常肺癌、肺癌、肝癌和宫颈癌细胞系中的表达水平。在肝癌、前列腺癌、肾癌和脑癌中，转录水平显著升高，而在结肠癌和肺癌中，转录水平显著下降。定量RT-PCR证实TSPO在正常肺细胞系中的表达水平高于肺癌细胞系。在大多数癌症中，TSPO mRNA表达水平的增加不一定是一个很好的诊断生物标志物，因为变化不够大，在原位杂交检测时不会有显着差异。
TSPO is a receptor involved in the regulation of cellular proliferation, apoptosis and mitochondrial functions. Previous studies showed that the expression of TSPO protein correlated positively with tumour malignancy and negatively with patient survival. The aim of this study was to determine the transcription of Tspo mRNA in various types of normal and cancer tissues. In situ hybridization was performed to localise the Tspo mRNA in various human normal and cancer tissues. The relative level of Tspo mRNA was quantified using fluorescent intensity and visual estimation of colorimetric staining. RT-PCR was used to confirm these mRNA levels in normal lung, lung cancer, liver cancer, and cervical cancer cell lines. There was a significant increase in the level of transcription in liver, prostate, kidney, and brain cancers while a significant decrease was observed in cancers of the colon and lung. Quantitative RT-PCR confirmed that the mRNA levels of Tspo are higher in a normal lung cell line than in a lung cancer cell line. An increase in the expression levels of Tspo mRNA is not necessarily a good diagnostic biomarker in most cancers with changes not being large enough to be significantly different when detected by in situ hybridisation.
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影响因子：
4.1
作者：
Batarseh, Amani;Papadopoulos, Vassilios
通讯作者：
Papadopoulos, Vassilios
DOI：
10.1016/j.biocel.2013.03.004
发表时间：
2013-07-01
影响因子：
4
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
2.8
作者：
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通讯作者：
Papadopoulos, V
影响因子：
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作者：
BUTLEN, D
通讯作者：
BUTLEN, D