Surface defects on plate-shaped silver nanoparticles contribute to its hazard potential in a fish gill cell line and zebrafish embryos.
Surface defects on plate-shaped silver nanoparticles contribute to its hazard potential in a fish gill cell line and zebrafish embryos.
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DOI：
10.1021/nn204671v
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发表时间：
2012-05-22
期刊：
影响因子：
17.1
通讯作者：
Nel AE
中科院分区：
文献类型：
--
作者：
George S;Lin S;Ji Z;Thomas CR;Li L;Mecklenburg M;Meng H;Wang X;Zhang H;Xia T;Hohman JN;Lin S;Zink JI;Weiss PS;Nel AE
我们调查和比较纳米大小的银球，板，电线在鱼鳃上皮细胞系（RT-W1）和斑马鱼胚胎了解机制的毒性工程纳米材料引起相当大的环境问题。虽然大多数Ag纳米颗粒在RT-W1中诱导N-乙酰半胱氨酸敏感的毒性氧化应激效应，但Ag纳米片比其他颗粒形状毒性更大。有趣的是，虽然Ag离子脱落和生物利用度未能解释纳米片的高毒性，但细胞损伤需要直接的颗粒接触，导致RT-W1以及红细胞（RBC）中的细胞膜溶解。银纳米片在斑马鱼胚胎中的毒性也要大得多，尽管它们将银脱落到暴露介质中的能力较低。为了阐明Ag纳米片的“表面反应性”，进行了高分辨率透射电子显微镜检查，并证明了纳米片表面上的高水平晶体缺陷（堆垛层错和点缺陷）。使用半胱氨酸的表面涂层来钝化表面缺陷，并证明在RT-W1细胞、RBC和斑马鱼胚胎中的毒性降低。这项研究表明，除了确定的作用，银离子脱落的球形纳米粒子的晶体缺陷的重要作用，有助于银纳米粒子的毒性。体外和体内毒理学评估之间的良好相关性说明了使用鱼细胞系与斑马鱼胚胎平行进行预测环境毒理学范例的实用性。
We investigated and compared nano-size Ag spheres, plates, and wires in a fish gill epithelial cell line (RT-W1) and in zebrafish embryos to understand the mechanism of toxicity of an engineered nanomaterial raising considerable environmental concern. While most of the Ag nanoparticles induced N-acetyl cysteine sensitive toxic oxidative stress effects in RT-W1, Ag nanoplates were considerably more toxic than other particle shapes. Interestingly, while Ag ion shedding and bioavailability failed to explain the high toxicity of the nanoplates, cellular injury required direct particle contact, resulting in cell membrane lysis in RT-W1 as well as red blood cells (RBC). Ag nanoplates were also considerably more toxic in zebrafish embryos in spite of their lesser ability to shed Ag into the exposure medium. In order to elucidate the “surface reactivity” of Ag nanoplates, high-resolution transmission electron microscopy was performed and demonstrated a high level of crystal defects (stacking faults and point defects) on the nanoplate surfaces. Surface coating with cysteine was used to passivate the surface defects and demonstrated a reduction of toxicity in RT-W1 cells, RBC, and zebrafish embryos. This study demonstrates the important role of crystal defects in contributing to Ag nanoparticle toxicity in addition to the established roles of Ag ion shed from spherical nanoparticles. The excellent correlation between the in vitro and in vivo toxicological assessment illustrates the utility of using a fish cell line in parallel with zebrafish embryos to perform a predictive environmental toxicological paradigm.
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影响因子：
15
作者：
George, Saji;Pokhrel, Suman;Ji, Zhaoxia;Henderson, Bryana L.;Xia, Tian;Li, LinJiang;Zink, Jeffrey I.;Nel, Andre E.;Maedler, Lutz
通讯作者：
Maedler, Lutz
影响因子：
19
作者：
Aherne, Damian;Ledwith, Deirdre M.;Kelly, John M.
通讯作者：
Kelly, John M.
影响因子：
17.1
作者：
George, Saji;Pokhrel, Suman;Xia, Tian;Gilbert, Benjamin;Ji, Zhaoxia;Schowalter, Marco;Rosenauer, Andreas;Damoiseaux, Robert;Bradley, Kenneth A.;Maedler, Lutz;Nel, Andre E.
通讯作者：
Nel, Andre E.
影响因子：
10.4
作者：
Fubini, B
通讯作者：
Fubini, B
影响因子：
3.2
作者：
LILIUS, H;SANDBACKA, M;ISOMAA, B
通讯作者：
ISOMAA, B