siRNA Delivery Using Dithiocarbamate-Anchored Oligonucleotides on Gold Nanorods.
siRNA Delivery Using Dithiocarbamate-Anchored Oligonucleotides on Gold Nanorods.
复制标题
DOI：
10.1021/acs.bioconjchem.8b00723
复制
发表时间：
2019-02-20
影响因子：
4.7
通讯作者：
Wei A
中科院分区：
文献类型：
--
作者：
Wang J;Thomas M;Lin P;Cheng JX;Matei DE;Wei A
We present a robust method for loading small interfering RNA (siRNA) duplexes onto the surfaces of gold nanorods (GNRs) at high density, using near-infrared (NIR) laser irradiation to trigger their intracellular release with subsequent knockdown activity. Citrate-stabilized GNRs were first coated with oleylsulfobetaine (OSB), a zwitterionic amphiphile with low cytotoxicity, which produced stable dispersions at high ionic strength. Amine-modified siRNA duplexes were converted into dithiocarbamate (DTC) ligands and adsorbed onto GNR surfaces in a single incubation step at 0.5 M NaCl, simplifying the charge screening process. The DTC anchors were effective at minimizing premature siRNA desorption and release, a common but often overlooked problem in the use of gold nanoparticles as oligonucleotide carriers. The activity of GNR‒siRNA complexes was evaluated systematically against an eGFP-producing ovarian cancer cell line (SKOV-3) using folate receptor-mediated uptake. Efficient knockdown was achieved by using a femtosecond-pulsed laser source to release DTC-anchored siRNA, with essentially no contributions from spontaneous (dark) RNA desorption. GNRs coated with thiol-anchored siRNA duplexes were less effective, and also permitted low levels of knockdown activity without photothermal activation. Optimized siRNA delivery conditions were applied toward the targeted knockdown of transglutaminase 2 (TG2), whose expression is associated with the progression of recurrent ovarian cancer, with a reduction in activity of over 80% achieved after a single pulsed laser treatment.
登录
查看更多内容
影响因子：
17.1
作者：
Leonov, Alexei P.;Zheng, Jiwen;Clogston, Jeffrey D.;Stern, Stephan T.;Patri, Anil K.;Wei, Alexander
通讯作者：
Wei, Alexander
影响因子：
3.9
作者：
Huff, Terry B.;Hansen, Matthew N.;Wei, Alexander
通讯作者：
Wei, Alexander
DOI：
10.2217/nnm.11.20
发表时间：
2011-06
期刊：
Nanomedicine (London, England)
影响因子：
--
作者：
Bonoiu AC;Bergey EJ;Ding H;Hu R;Kumar R;Yong KT;Prasad PN;Mahajan S;Picchione KE;Bhattacharjee A;Ignatowski TA
通讯作者：
Ignatowski TA
影响因子：
--
作者：
Ding L;Sun R;Zhang X
通讯作者：
Zhang X
影响因子：
3.3
作者：
Hansen, Matthew N.;Chang, Ling-Shao;Wei, Alexander
通讯作者：
Wei, Alexander