TaARPC3, Contributes to Wheat Resistance against the Stripe Rust Fungus.
TaARPC3, Contributes to Wheat Resistance against the Stripe Rust Fungus.
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TaARPC3，有助于小麦抵抗条锈菌
DOI：
10.3389/fpls.2017.01245
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发表时间：
2017
影响因子：
5.6
通讯作者：
Ma Q
中科院分区：
文献类型：
--
作者：
Qi T;Wang J;Sun Q;Day B;Guo J;Ma Q
肌动蛋白细胞骨架参与许多细胞过程，包括较少表征的过程，如核组织、染色质重塑、转录和信号转导。肌动蛋白相关蛋白2/3复合物（actin related protein 2/3 complex，Arp 2/3 complex）是肌动蛋白细胞骨架动力学的关键调节因子，在多种组织和细胞类型中控制着多种发育过程。迄今为止，Arp 2/3复合物在植物抗病信号传导中的作用在很大程度上是未知的。在此，我们鉴定并表征了小麦ARPC 3，TaARPC 3，其编码Arp 2/3复合物的C3亚基。TaARPC 3在酿酒酵母Δ arc 18突变体arc 18中的表达导致了S.酿酒酵母，以及恢复野生型细胞形状畸形。TaARPC 3在小麦原生质体中瞬时表达时主要定位于细胞核和细胞质中。TaARPC 3对小麦条锈菌无毒小种的诱导作用显著。小麦种（Pst）通过病毒诱导的基因沉默敲低TaARPC 3导致通过肌动蛋白细胞骨架组织的特异性减少而降低对Pst的抗性。有趣的是，发现这种减少与活性氧（ROS）积累，过敏反应（HR），TaCAT 1 mRNA积累增加和Pst生长的阻滞一致。总之，这些发现表明，TaARPC 3是Arp 2/3复合物的关键亚基，该复合物是小麦抗Pst所需的，该过程与肌动蛋白细胞骨架的调节有关。
The actin cytoskeleton participates in numerous cellular processes, including less-characterized processes, such as nuclear organization, chromatin remodeling, transcription, and signal transduction. As a key regulator of actin cytoskeletal dynamics, the actin related protein 2/3 complex (Arp2/3 complex) controls multiple developmental processes in a variety of tissues and cell types. To date, the role of the Arp2/3 complex in plant disease resistance signaling is largely unknown. Herein, we identified and characterized wheat ARPC3, TaARPC3, which encodes the C3 subunit of the Arp2/3 complex. Expression of TaARPC3 in the arc18 mutant of Saccharomyces cerevisiae Δarc18 resulted in complementation of stress-induced phenotypes in S. cerevisiae, as well as restore wild-type cell shape malformations. TaARPC3 was found predominantly to be localized in the nucleus and cytoplasm when expressed transiently in wheat protoplast. TaARPC3 was significantly induced in response to avirulent race of Puccinia striiformis f. sp. tritici (Pst). Knock-down of TaARPC3 by virus-induced gene silencing resulted in a reduction of resistance against Pst through a specific reduction in actin cytoskeletal organization. Interestingly, this reduction was found to coincide with a block in reactive oxygen species (ROS) accumulation, the hypersensitive response (HR), an increase in TaCAT1 mRNA accumulation, and the growth of Pst. Taken together, these findings suggest that TaARPC3 is a key subunit of the Arp2/3 complex which is required for wheat resistance against Pst, a process that is associated with the regulation of the actin cytoskeleton.
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DOI：
10.1038/nrm2867
发表时间：
2010-04
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
--
影响因子：
7.5
作者：
Chesarone MA;Goode BL
通讯作者：
Goode BL
影响因子：
14.9
作者：
Chenna, R;Sugawara, H;Thompson, JD
通讯作者：
Thompson, JD
影响因子：
4.3
作者：
Eun, SO;Bae, SH;Lee, Y
通讯作者：
Lee, Y
DOI：
10.1083/jcb.200310148
发表时间：
2004-03-15
期刊：
The Journal of cell biology
影响因子：
--
作者：
Gourlay CW;Carpp LN;Timpson P;Winder SJ;Ayscough KR
通讯作者：
Ayscough KR