Assessment of tissue distribution and concentration of β-cryptoxanthin in response to varying amounts of dietary β-cryptoxanthin in the Mongolian gerbil
Assessment of tissue distribution and concentration of β-cryptoxanthin in response to varying amounts of dietary β-cryptoxanthin in the Mongolian gerbil
复制标题
蒙古沙鼠中β-隐黄质的组织分布和浓度对不同膳食β-隐黄质含量的响应评估
DOI：
10.1017/s0007114513003371
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发表时间：
2013
影响因子：
3.6
通讯作者：
B. Burri
中科院分区：
文献类型：
--
作者：
Michael R. La Frano;Chenghao Zhu;B. Burri
关于维生素原A类胡萝卜素β-隐黄质的吸收和组织储存的知识普遍缺乏。本研究以长爪沙鼠（Meriones unguiculatus）为动物模型，研究了β-隐黄素在人维生素A原类胡萝卜素代谢中的全身组织分布。在类胡萝卜素耗尽5天后，对5只沙鼠实施安乐死以进行基线测量。其余沙鼠被置于三个体重匹配的治疗组（n 8）。所有组均接受20 μg/d的浓缩β-隐黄素，而第二和第三组分别接受20和40 μg/d的纯β-隐黄素（CX 40和CX 60），共21 d。在研究的最后2天，收集每个处理组两只沙鼠的尿液和粪便样品。在全血中检测到β-隐黄素，并在14个组织中的12个中进行了分析。大多数组织与肝脏相似，其中CX 60组（17.8（sem 0.7）μg/器官; P= 0.004）和CX 40组（16.2（sem 0.9）μg/器官; P= 0.006）中的β-隐黄素浓度显著高于CX 20组（13.3（sem 0.4）μg/器官）。然而，在肠组织中，β-隐黄素的浓度仅在CX 60组中增加。尽管由于研究前饮食含有高水平的维生素A，在基线时组织中的维生素A浓度升高，但β-隐黄素维持了这些维生素A储存。这些结果表明，β-隐黄素储存在许多组织中，可能表明其功能是广泛的。
There is a general lack of knowledge regarding the absorption and tissue storage of the provitamin A carotenoid β-cryptoxanthin. The present study investigated the whole-body tissue distribution of β-cryptoxanthin in an appropriate small animal model, the Mongolian gerbil (Meriones unguiculatus), for human provitamin A carotenoid metabolism. After 5 d of carotenoid depletion, five gerbils were euthanised for baseline measurements. The remaining gerbils were placed in three weight-matched treatment groups (n 8). All the groups received 20 μg/d of β-cryptoxanthin from tangerine concentrate, while the second and third groups received an additional 20 and 40 μg/d of pure β-cryptoxanthin (CX40 and CX60), respectively, for 21 d. During the last 2 d of the study, urine and faecal samples of two gerbils from each treatment group were collected. β-Cryptoxanthin was detected in the whole blood, and in twelve of the fourteen tissues analysed. Most tissues resembled the liver, in which the concentrations of β-cryptoxanthin were significantly higher in the CX60 (17·8 (sem 0·7) μg/organ; P= 0·004) and CX40 (16·2 (sem 0·9) μg/organ; P= 0·006) groups than in the CX20 group (13·3 (sem 0·4) μg/organ). However, in intestinal tissues, the concentrations of β-cryptoxanthin increased only in the CX60 group. Despite elevated vitamin A concentrations in tissues at baseline due to pre-study diets containing high levels of vitamin A, β-cryptoxanthin maintained those vitamin A stores. These results indicate that β-cryptoxanthin is stored in many tissues, potentially suggesting that its functions are widespread.
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影响因子：
4.2
作者：
Grune, Tilman;Lietz, Georg;Biesalski, Hans K.
通讯作者：
Biesalski, Hans K.
影响因子：
4.2
作者：
During, Alexandrine;Dawson, Harry D.;Harrison, Earl H.
通讯作者：
Harrison, Earl H.
影响因子：
3.9
作者：
Mein JR;Dolnikowski GG;Ernst H;Russell RM;Wang XD
通讯作者：
Wang XD
影响因子：
6.5
作者：
During, A;Hussain, MM;Harrison, EH
通讯作者：
Harrison, EH
小剂量的 14C-{β}-胡萝卜素在人体中被吸收并转化为类维生素A。
DOI：
10.3945/jn.109.105114
发表时间：
2009
期刊：
The Journal of nutrition
影响因子：
--
作者：
Ho,CharleneC;deMoura,FabianaF;Kim,Seung-Hyun;Burri,BettyJ;Clifford,AndrewJ
通讯作者：
Clifford,AndrewJ