Immunohistochemistry and in situ hybridization in the developing chicken brain.
Immunohistochemistry and in situ hybridization in the developing chicken brain.
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发育中鸡脑的免疫组织化学和原位杂交。
DOI：
10.1007/978-1-62703-655-9_15
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Gong,Qizhi
中科院分区：
文献类型：
--
作者：
Tucker,RichardP;Gong,Qizhi
One of the first steps in studies of gene function is the spatiotemporal analysis of patterns of gene expression. Indirect immunohistochemistry is a method that allows the detection of a protein of interest by incubating a histological section with an antibody or antiserum raised against the protein and then localizing this primary antibody with a tagged secondary antibody. To determine the cellular source of a protein of interest, or if a specific antibody is not available, specific transcripts can be localized using in situ hybridization. A histological section is incubated with a labeled RNA probe that is complementary to the target transcript; after hybridization with the target transcript, the labeled RNA probe can be identified with an antibody. Here we describe materials and methods used to perform basic indirect immunohistochemistry and in situ hybridization on frozen sections through the developing chicken brain, emphasizing controls and potential problems that may be encountered.
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